抗体

Keratin, LMW (Low Molecular Weight Keratin, Type 1 Keratin, Type 1 Cytokeratin, Acidic Keratin, Acidic Cytokeratin, Epithelial Type 1 Keratin)

138561

20微克

353美元

单克隆

小鼠

未共轭

AE-1

Keratin, LMW (Low Molecular Weight Keratin, Type 1 Keratin, Type 1 Cytokeratin, Acidic Keratin, Acidic Cytokeratin, Epithelial Type 1 Keratin)

小鼠

单克隆抗体

抗体

20微克

353美元

AE-1

IgG1, k

This monoclonal antibody recognizes the 56.5kD (CK10); 50kD (CK14); 50kD (CK15); 48kD (CK16); 40kD (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kD. Monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kD keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Skin, Squamous cell carcinoma (SCC) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Ca Ch Hu Mk Mo Rb Rt

Human epidermal keratin

Recognizes human acidic (type 1, epithelial) keratins/cytokeratins. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat, chicken and turtle.

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138561-AF.

~0.2毫克/毫升

Product Type: Mab Isotype: IgG1,k Clone No: AE-1 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138561-AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Human epidermal keratin Specificity: Recognizes human acidic (type 1, epithelial) keratins/cytokeratins. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat, chicken and turtle. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF免疫组化免疫印迹

-20°C

1. Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8. 2. Tseng SCG et. al. Cell 1982; 30361.

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