抗体

p53(肿瘤抑制蛋白, 癌基因蛋白)

138582

20微克

353美元

单克隆

小鼠

未共轭

BP53-12

p53(肿瘤抑制蛋白, 癌基因蛋白)

小鼠

单克隆抗体

抗体

20微克

353美元

BP53-12

IgG2a

Recognizes a 53kD protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. Its epitope maps within the N-terminus (aa20-25) of p53 oncoprotein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, re plication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: MDA-MB-231 Cells. Breast or Colon carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Full length recombinant protein corresponding to human p53.

Recognizes human p53. Does not cross-react with mouse or rat.

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138582-AF.

~0.2毫克/毫升

Product Type: Mab Isotype: IgG2a Clone No: BP53-12 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138582AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Full length recombinant protein corresponding to human p53. Specificity: Recognizes human p53. Does not cross-react with mouse or rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF免疫组化免疫沉淀免疫印迹

-20°C

1. Bartek J et. al. Journal of Pathology, 1993, 169(1):27-34.

https://usbio-images.r.worldssl.net/prodimages/11/138582_1jpeg