抗体

Podocalyxin (Podocalyxin-like Protein, PDXL)

138585

20微克

353美元

单克隆

小鼠

未共轭

3D3

Podocalyxin (Podocalyxin-like Protein, PDXL)

小鼠

单克隆抗体

抗体

20微克

353美元

3D3

IgG1

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidne y development and homeostasis, blood filtration and urine formation. It is also expressed on va scular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturi a) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1-2ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunoprecipitation: 1-2ug/500ug protein Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Rb Rt

Recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin. Epitope: extracellular domain

Recognizes human podocalyxin. Species Crossreactivity: rabbit and rat.

纯化

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138585-AF.

~0.2毫克/毫升

Product Type: Mab Isotype: IgG1 Clone No: 3D3 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138585AF. Purity: Purified Immunogen: Recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin. Epitope: extracellular domain Specificity: Recognizes human podocalyxin. Species Crossreactivity: rabbit and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF免疫组化免疫沉淀免疫印迹

-20°C

1. Kershaw DB et. al . J Biol Chem 272:15708-15714 (1997). 2. Sassetti C et. al. J Exp Med 187:1965-1975. 3. Nielsen JS et. al. J Am Soc Nephrol 20:1669-1676 (2009).

https://usbio-images.r.worldssl.net/prodimages/29/138585_1jpeg