产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Akt, phosphorylated (Ser473) (Rac PKa, PKBa)
目录 :
A1124-02T
规格 :
20微升
价格 :
355美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
克隆名称 :
10g137(193H12)
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
A1124-02T
Product wo Prefix :
Akt, phosphorylated (Ser473) (Rac PKa, PKBa)
宿主 :
产品类型 :
单克隆抗体
抗原修饰 :
磷酸化
类别 :
抗体
尺寸1 :
20微升
Size1 List :
355美元
克隆#USB :
10g137(193H12)
抗体亚型 :
IgG
Desc1 :
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9) and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3alpha and beta (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3beta mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18). Inhibition of mTOR stops the protein synthesis machinery by inactivating p70 S6 kinase and activating the eukaryotic initiation factor 4E binding protein 1 (4E-BP1), an inhibitor of translation (18,19). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:200 Flow Cytometry: 1:400 Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu Mo Rt
免疫原 :
Synthetic phosphopeptide corresponding to residues around Ser473 of mouse Akt.
特异性 :
Recognizes endogenous levels of Akt only when phosphorylated at Ser473. Species crossreactivity: human, mouse and rat.
纯度 :
纯化
形式 :
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Mab Isotype: IgG Clone No: 10g137(193H12) Host: rabbit Source: human Concentration: Not determined. Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues around Ser473 of mouse Akt. Specificity: Recognizes endogenous levels of Akt only when phosphorylated at Ser473. Species crossreactivity: human, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC IF免疫沉淀免疫印迹
储存温度 :
-20°C
参考文献 :
1. Franke, T.F. et al. (1997) Cell 88, 435-7. 2. Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602. 3. Franke, T.F. et al. (1995) Cell 81, 727-36. 4. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51. 5. Sarbassov, D.D. et al. (2005) Science 307, 1098-101. 6. Jacinto, E. et al. (2006) Cell 127, 125-37. 7. Cardone, M.H. et al. (1998) Science 282, 1318-21. 8. Brunet, A. et al. (1999) Cell 96, 857-68. 9. Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4. 10. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. 11. Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8. 12. Hajduch, E. et al. (2001) FEBS Lett 492, 199-203. 13. Cross, D.A. et al. (1995) Nature 378, 785-9. 14. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511. 15. Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30. 16. Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52. 17. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. 18. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57. 19. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/32/A1124-02T_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。