抗体

Akt, pan (Rac PKa, PKBa)

A1124-12A

100微升

516美元

单克隆

domestic rabbit

未共轭

10g141(11E7)

Akt, pan (Rac PKa, PKBa)

兔

单克隆抗体

抗体

100微升

516美元

10g141(11E7)

IgG

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9) and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3alpha and beta (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3beta mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18). Inhibition of mTOR stops the protein synthesis machinery by inactivating p70 S6 kinase and activating the eukaryotic initiation factor 4E binding protein 1 (4E-BP1), an inhibitor of translation (18,19). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:100 Flow Cytometry: 1:100 Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide corresponding to the C-terminal sequence of mouse Akt.

Recognizes endogenous levels of total mouse Akt. Does not cross-react with other related proteins Species crossreactivity: human, rat and monkey.

纯化

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

未确定

Product Type: Mab Isotype: IgG Clone No: 10g141(11E7) Host: rabbit Source: mouse Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to the C-terminal sequence of mouse Akt. Specificity: Recognizes endogenous levels of total mouse Akt. Does not cross-react with other related proteins Species crossreactivity: human, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF免疫组化免疫沉淀免疫印迹

-20°C

https://usbio-images.r.worldssl.net/prodimages/18/A1124-12A_1.jpg

Immunohistochemical analysis of paraffin-embedded human melanoma, using A1124-12A.

https://usbio-images.r.worldssl.net/prodimages/18/A1124-12A_5.jpg

Immunohistochemical analysis using A1124-12A

https://usbio-images.r.worldssl.net/prodimages/18/A1124-12A_6.jpg

Immunohistochemical analysis of paraffin-embedded HeLa cells untreated (left) or transfected (right), using A1124-12A (top) or Cleaved Caspase-3 (Asp175) (bottom). Note the induction of cleaved caspase-3 in Akt deficient cells.

https://usbio-images.r.worldssl.net/prodimages/18/A1124-12A_7.pct

Flow cytometric analysis of untreated Jurkat cells, using A1124-12A (blue) compared to a nonspecific negative control antibody (red).