产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Bromodeoxyuridine (BrdU) discontinued
目录 :
B2850-01B
规格 :
200微克
价格 :
689美元
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
3H579
更多信息或购买 :
产品信息
目录号 :
B2850-01B
Product wo Prefix :
Bromodeoxyuridine (BrdU) discontinued
宿主 :
大鼠
产品类型 :
单克隆抗体
类别 :
抗体
尺寸1 :
200微克
Size1 List :
689美元
克隆#USB :
3H579
抗体亚型 :
IgG2a
Desc1 :
BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:25-1:200. Use 20ul to label 10e6 cells in 100ul Immunohistochemistry (Paraffin): 1:25-1:200 Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1904-49H IgG, F(ab’)2, H&L X-Adsorbed (HRP) RbxRt I1904-49U IgG, F(ab’)2, H&L X-Adsorbed (FITC) RbxRt Recommended Negative Controls: I1904-83M1: IgG2a, Rat Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
免疫原 :
溴脱氧尿苷(溴化去氧尿苷)
特异性 :
Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Does not crossreact with thymidine but does react weakly with chlorodeoxyuridine.
纯度 :
Purified by Protein G affinity chromatography.
形式 :
以溶解在PBS液体方式提供, 0.09% 叠氮化钠
浓度 :
~1毫克/毫升
Desc2 :
Product Type: Mab Isotype: IgG2a Clone No: 3H579 Host: rat Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, 0.09% sodium azide. Purity: Purified by Protein G affinity chromatography. Immunogen: Bromodeoxyuridine (BrdU) Specificity: Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Does not crossreact with thymidine but does react weakly with chlorodeoxyuridine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC免疫组化
储存温度 :
-20°C
参考文献 :
1. Vanderlaan, M. & Thomas, C.B. (1985) Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry. 6: 501-505. 2. Ghiringelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation. J. Exp. Med. 202: 919-929. 3. Dolbeare, F. (1995) Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics. Histochem. J. 27: 339-369.
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。