产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
胱氨酸-天冬氨酸蛋白酶2(ICH-1, Nedd2)
目录 :
C2086-72D
规格 :
100微升
价格 :
516美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
4i13(C2)
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
C2086-72D
Product wo Prefix :
胱氨酸-天冬氨酸蛋白酶2(ICH-1, Nedd2)
宿主 :
小鼠
产品类型 :
单克隆抗体
类别 :
抗体
尺寸1 :
100微升
Size1 List :
516美元
克隆#USB :
4i13(C2)
抗体亚型 :
IgG1
Desc1 :
Caspase-2 (Nedd2/ICH-1) is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved at Asp316, producing a 14kD fragment and a 32kD prodomain/large subunit. Subsequent processing at Asp152 and Asp330 produces an 18kD large subunit and a 12kD small fragment (1). Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD (2). This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events (3, 4). In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase- 3 activation, extending a possible role for caspase- 2 as a parallel effector caspase (5). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu
免疫原 :
Synthetic peptide corresponding to the C-terminal portion of human caspase-2.
特异性 :
Recognizes endogenous levels of human procaspase-2 at ~48kD as well as its 14 and 12kD cleaved fragments.
纯度 :
腹水
形式 :
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Mab Isotype: IgG1 Clone No: 4i13(C2) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic peptide corresponding to the C-terminal portion of human caspase-2. Specificity: Recognizes endogenous levels of human procaspase-2 at ~48kD as well as its 14 and 12kD cleaved fragments. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E免疫印迹
储存温度 :
-20°C
参考文献 :
(1) Li, H. et al. (1997) J. Biol. Chem 272, 21010–21017. 2) Tinel, A. and Tschopp, J. (2004) Science 304, 843–846. 3) Dirsch, V.M. et al. (2004) Oncogene 23, 1586–1593. 4) Castedo, M. et al. (2004) Oncogene (in press). 5) Paroni, G. et al. (2001) J. Biol. Chem. 276, 21907–21915.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/22/C2086-72D_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。