抗体

Caspase 9 (ICE-LAP6, Mch6, Apaf 3)

C2088-01D

100微升

516美元

单克隆

小鼠

未共轭

4i14(C9)

Caspase 9 (ICE-LAP6, Mch6, Apaf 3)

小鼠

单克隆抗体

抗体

100微升

516美元

4i14(C9)

IgG1

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47kD procaspase-9/Apaf 1. This complex processes procaspase-9 into a large active fragment (35kD or 17kD) and a small fragment (10kD) by self-cleavage at Asp315 (3-5). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (6–9). In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at Asp330. This process serves as positive feedback to amplify the apoptotic signal in the caspase activation pathway (3–5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hm Hu Mk Mo Rt

Recombinant protein corresponding to human caspase-9.

Detects endogenous levels of the pro form of caspase-9 as well as cleaved fragments. Human: 47/37/35kD and mouse and rat: 51/39/37kD. Species Crossreactivity: Human, mouse, monkey, hamster and rat.

腹水

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

未确定

Product Type: Mab Isotype: IgG1 Clone No: 4i14(C9) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Ascites Immunogen: Recombinant protein corresponding to human caspase-9. Specificity: Detects endogenous levels of the pro form of caspase-9 as well as cleaved fragments. Human: 47/37/35kD and mouse and rat: 51/39/37kD. Species Crossreactivity: Human, mouse, monkey, hamster and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫印迹

-20°C

(1) Duan, H. et al. (1996) J. Biol. Chem. 271, 16720–16724.(2) Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099–27106.(3) Liu, X. et al. (1996) Cell 86, 147–157.(4) Li, P. et al. (1997) Cell 91, 479–489.(5) Zou, H. et al. (1999) J. Biol. Chem. 274, 11549–11556.(6) Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215–2223.(7) Slee, E. A. et al. (1999) J. Cell Biol. 144, 281–292.(8) Sun, X. et al. (1999) J. Biol. Chem. 274, 5053–5060.(9) MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469–479.

https://usbio-images.r.worldssl.net/prodimages/25/C2088-01D_1.jpg