抗体

Caspase 9, Cleavage Site (Asp330) (ICE-LAP6, Mch6) discontinued

C2088-08B

100微升

538美元

多克隆

domestic rabbit

未共轭

Caspase 9, Cleavage Site (Asp330) (ICE-LAP6, Mch6) discontinued

兔

多克隆抗体

抗体

100微升

538美元

IgG

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47kD)/Apaf 1. This complex processes procaspase-9 into a large active subunit (35kD or 17kD) and a small subunit (10kD) by self-cleavage at Asp315 (3–5). Cleaved caspase-9 further processes other caspase members, including caspase- 3 and caspase-7, to initiate a caspase cascade, leading to programmed cell death (6–9). In addition to self-cleavage, procaspase-9 also can be cleaved in vivo by caspase-3 at Asp330. This process serves as positive feedback to amplify the apoptotic signal in the caspase activation pathway (3–5). Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunocytochemistry (ABC) 1:100 Flow cytometry 1:10 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic peptide corresponding to amino terminus residues adjacent to Asp330 of human Caspase 9.

Recognizes endogenous levels of the large fragment (37kD with prodomain/17kD) of human Caspase 9 following cleavage at Asp330. Does not recognize the uncleaved procaspase 9.

Purified by Protein A and peptide affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

未确定

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to amino terminus residues adjacent to Asp330 of human Caspase 9. Specificity: Recognizes endogenous levels of the large fragment (37kD with prodomain/17kD) of human Caspase 9 following cleavage at Asp330. Does not recognize the uncleaved procaspase 9. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫沉淀免疫印迹

-20°C

1. Duan, H. et al. (1996) J. Biol. Chem. 271:16720-16724. 2. Srinivasula, S.M. et al. (1996) J. Biol. Chem. 271:27099-27106. 3. Liu, X. et al. (1996) Cell 86:147-157. 4. Li, P. et al. (1997) Cell 91:479-489. 5. Zou, H. et al. (1999) J. Biol. Chem. 274:11549-11556. 6. Deveraux, Q.L. et al. (1998) EMBO J. 17: 2215-2223. 7. Slee, E.A. et al. (1999) J. Cell. Biol. 144:281-292. 8. Sun, X. et al. (1999) J. Biol. Chem. 274:5053-5060. 9. MacFarlane, M. et al. (1997) J. Cell. Biol. 137:469-479.

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