产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
CD95(肿瘤坏死因子受体超家族成员6)
目录 :
C2442-21E
规格 :
20微升
价格 :
342美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
C2442-21E
Product wo Prefix :
CD95(肿瘤坏死因子受体超家族成员6)
宿主 :
兔
产品类型 :
多克隆抗体
类别 :
抗体
尺寸1 :
20微升
Size1 List :
342美元
抗体亚型 :
IgG
Desc1 :
Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenophathy and splenomegaly as a result of decreased apoptosis in CD4-CD8-T lymphocytes (3,4). FasL (CD95L, Apo-1L) is a type 2 membrane protein of 280aa (runs at ~40kD upon glycosylation) that belongs to the TNF family which also includes TNF-alpha, TRAIL and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to the execution of apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells and at immunologically privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26kD trimeric soluble form (8,9).
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunohistochemistry (paraffin): 1:480
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Hu
免疫原 :
Synthetic peptide corresponding to residues surrounding Lys259 of human Fas, within the intracellular region.
特异性 :
Recognizes endogenous levels of total human Fas protein.
纯度 :
纯化
形式 :
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Pab
Isotype: IgG
Host: rabbit
Source: human
Concentration: Not determined
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity: Purified
Immunogen: Synthetic peptide corresponding to residues surrounding Lys259 of human Fas, within the intracellular region.
Specificity: Recognizes endogenous levels of total human Fas protein.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
免疫组化免疫印迹
储存温度 :
-20°C
参考文献 :
1. Suda, T. et al. (1993) Cell 75:1169–78. 2. Lee, H.O. and Ferguson, T.A. (2003) Cytokine Growth Factor Rev 14:325–35. 3. Watanabe-Fukunaga, R. et al. (1992) Nature 356:314–7. 4. Hahne, M. et al. (1995) Int Immunol 7:1381–6. 5. Nagata, S. (1997) Cell 88:355–65. 6. Green, D.R. and Ferguson, T.A. (2001) Nat Rev Mol Cell Biol 2:917–24. 7. Walker, P.R. et al. (1997) J Immunol 158:4521–4. 8. Kayagaki, N. et al. (1995) J Exp Med 182:1777–83. 9. Tanaka, M. et al. (1995) EMBO J 14:1129–35.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/14/C2442-21E_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
tech@usbio.net
https://www.usbio.net800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。