抗体

Crk II, phosphorylated (Tyr221)

C7924-50

100微升

566美元

多克隆

domestic rabbit

未共轭

磷酸化

Crk II, phosphorylated (Tyr221)

兔

多克隆抗体

磷酸化

抗体

100微升

566美元

IgG

CrkII, a cellular homologue of v-Crk, belongs to a family of adaptor proteins with an SH2-SH3-SH3 domain structure that transmits signals from tyrosine kinases (1). The primary function of Crk is to recruit cytoplasmic proteins in the vicinity of tyrosine kinases through SH2-phospho-tyrosine interaction. Thus, the output from Crk depends on the SH3-binding proteins, which include the C3G and Sos guanine nucleotide exchange proteins, Abl tyrosine kinase, DOCK180 and some STE20-related kinases. The variety of Crk-binding proteins indicates the pleiotropic function of Crk (2). The two CrkII SH3 domains are separated by a 54 amino acid linker region, which is highly conserved in Xenopus, chicken and mamalian CrkII proteins (3). Tyrosine 221 in this region is phosphorylated by the Abl tyrosine kinase (4), IGF-I receptor (5) and EGF receptor (6). Once Tyr221 is phosphorylated, CrkII undergoes a change in intramolecular folding and SH2-pTyr interaction, which causes rapid dissociation of CrkII from the tyrosine kinase complex (3). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Note: for Western Blots incubate membrane with diluted antibody in 5% BSA, 1X TBS, and 0.1% Tween-20 at 4ºC with gentle shaking overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hm Hu

Synthetic phosphopeptide corresponding to residues surrounding Tyr221 of human CrkII. Species sequence homology: mouse and rat 100%.

Detects endogenous levels of CrkII only when phosphorylated at tyrosine 221 at 42kD. Crossreactivity: Tyr207 phosphorylated CrkL. Does not crossract wth other tyrosine-phosphorylated proteins. Species Crossreactivity: Human and hamster.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

未确定

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr221 of human CrkII. Species sequence homology: mouse and rat 100%. Specificity: Detects endogenous levels of CrkII only when phosphorylated at tyrosine 221 at 42kD. Crossreactivity: Tyr207 phosphorylated CrkL. Does not crossract wth other tyrosine-phosphorylated proteins. Species Crossreactivity: Human and hamster. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E免疫印迹

-20°C

1. Zvara, A. et al. 2001, Oncogene 20, 951-961. 2. Kiyokawa, E. et al. 1997, Crit. Rev. Oncog. 8, 329-342. 3. Rosen, S. K. et al. 1995, Nature 374, 477-479. 4. Amoui, M. and Miller, W.T. 2000, Cell. Signal. 12, 637-643. 5. Koval, A. P. et al. 199,. Biochem. J. 330, 923-932. 6. Hashimoto, Y. et al. 1998, J. Biol. Chem. 273, 17186-17191.

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