产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Epidermal Growth Factor Receptor (erbB, EGFR) (APC)
目录 :
E3375-01B1
规格 :
100Tests
价格 :
692美元
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
APC
克隆名称 :
8H184
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
E3375-01B1
Product wo Prefix :
Epidermal Growth Factor Receptor (erbB, EGFR) (APC)
宿主 :
大鼠
产品类型 :
单克隆抗体
共轭标签 :
APC
类别 :
抗体
尺寸1 :
100Tests
Size1 List :
692美元
克隆#USB :
8H184
抗体亚型 :
IgG2a
Desc1 :
The epidermal growth factor receptor (EGF R) subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins that have an extracellular domain containing two cysteine-rich domains separated by a spacer region that is involved in ligand-binding, and a cytoplasmic domain that has a membrane-proximal tyrosine kinase domain, and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGFR gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGF R has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, heparin-binding EGF, and neuregulin-2 alpha, in the absence of a co-receptor. Ligand binding induces EGF R homodimerization as well as heterdimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation, and cell signaling. EGF R can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGF R signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGF R signaling has also been shown to play a role in carcinogenesis.1-3 Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Calc Crossreactivity :
Hu
又名 :
EC=2.7。 10.1, ERBB1, MENA
免疫原 :
Recombinant protein corresponding to Leu25-Ser645 from human EGF R/ErbB1, expressed in Mouse myeloma cell line NS0.
特异性 :
识别人类表皮生长因子受体
纯度 :
Purified by Protein G affinity chromatography from cell culture supernatant.
形式 :
Supplied as a liquid in saline, BSA, sodium azide. Labeled with APC.
浓度 :
未确定
Desc2 :
Product Type: Mab Isotype: IgG2a Clone No: 8H184 Host: rat Source: human Concentration: Not determined Form: Supplied as a liquid in saline, BSA, sodium azide. Labeled with APC. Purity: Purified by Protein G affinity chromatography from cell culture supernatant. Immunogen: Recombinant protein corresponding to Leu25-Ser645 from human EGF R/ErbB1, expressed in Mouse myeloma cell line NS0. Specificity: Recognizes human EGFR. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC
储存温度 :
4°C不冻结
参考文献 :
1. Daly, R.J., (1999) Growth Factors, 16:255-263. 2. Schlessinger, J., (2000) Cell. 103:211-225. 3. Maihle, N.J. et al., (2002) Cancer Treat. Res. 107:247-258.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/41/E3375-01B1_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。