产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Epidermal Growth Factor Receptor (erbB, EGFR), phosphorylated (Tyr992)
目录 :
E3375-53C
规格 :
100微升
价格 :
566美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
E3375-53C
Product wo Prefix :
Epidermal Growth Factor Receptor (erbB, EGFR), phosphorylated (Tyr992)
宿主 :
产品类型 :
多克隆抗体
抗原修饰 :
磷酸化
类别 :
抗体
尺寸1 :
100微升
Size1 List :
566美元
抗体亚型 :
IgG
Desc1 :
Epidermal growth factor (EGF) receptor is a 170kD single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation (1,2). Tyrosine 845 resides in the activation loop of the receptor kinase domain (3,4). Phosphorylation of Tyr845 may stabilize the activation loop, maintain the enzyme in an active state and provide a binding surface for substrate proteins. It has been shown that c-Src is involved in phosphorylation of the EGF receptor on Tyr845, which is associated with modulation of receptor function (5). Phospho-tyrosine 992 of activated EGF receptor is a direct binding site for the phospholipase C-gamma (PLCgamma) SH2 domain (6). This binding results in activation of PLC-gamma-mediated downstream signaling. Phosphorylation of Tyr1045 creates a major docking site for c-Cbl (7). Binding of c-Cbl to the activated EGF receptor leads to assembly of ubiquitination machinery to the receptor, and enables receptor ubiquitination and degradation (8). Phospho-tyrosine 1068 of activated EGF receptor is a direct binding site for the Grb2/SH2 domain (9). This binding results in Ras activation through a Grb2/Sos-1 signaling mechanism (2). Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:100. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Hu
免疫原 :
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr992 of human EGF receptor.
特异性 :
Recognizes human overexpressed EGF receptor only when phosphorylated at Tyr992 at 175kD. Does not crossreact with other phosphorylated receptor tyrosine kinases. Species sequence homology: Mouse and rat-100%.
纯度 :
Purified by Protein A affinity chromatography.
形式 :
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
浓度 :
未确定
Desc2 :
Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr992 of human EGF receptor. Specificity: Recognizes human overexpressed EGF receptor only when phosphorylated at Tyr992 at 175kD. Does not crossreact with other phosphorylated receptor tyrosine kinases. Species sequence homology: Mouse and rat-100%. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E免疫组化免疫印迹
储存温度 :
-20°C
参考文献 :
1. Hackel, P.O. et al. (1999) Curr. Opin. Cell Biol. 11, 184-189. 2. Zwick , E. et al. (1999) Trends Pharmacol. Sci. 20, 408-412. 3. Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-1054. 4. Hubbard, S.R. et al. (1994) Nature 372, 746-754. 5. Biscardi, J.S. et al. (1999) J. Biol. Chem. 274, 8335-8343. 6. Emlet, D.R. et al. (1997) J. Biol. Chem. 272, 4079-4086. 7. Levkowitz, G. et al. (1999) Mol. Cell 4, 1029-1040. 8. Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-1866. 9. Rojas, M. et al. (1996) J. Biol. Chem. 271, 27456-27461. 10. Iordanov, M. S. et al. (2002) Mol. Cell. Biol. 22, 5380-5394.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/11/E3375-53C_1.jpg
Picture 6 File Name :
https://usbio-images.r.worldssl.net/prodimages/11/E3375-53C_6.jpg
Picture6文本 :
Immunofluorescent staining of untreated (left) and EGF treated (right) A431 cells, using E3375-53C.
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。