产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Epidermal Growth Factor Receptor, phosphorylated (Tyr1173) (erbB, EGFR)
目录 :
E3375-55
规格 :
100微升
价格 :
571美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
克隆名称 :
4i5(53A5)
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
E3375-55
Product wo Prefix :
Epidermal Growth Factor Receptor, phosphorylated (Tyr1173) (erbB, EGFR)
宿主 :
产品类型 :
单克隆抗体
抗原修饰 :
磷酸化
类别 :
抗体
尺寸1 :
100微升
Size1 List :
571美元
克隆#USB :
4i5(53A5)
抗体亚型 :
IgG
Desc1 :
Epidermal growth factor (EGF) receptor is a 170kD tyrosine kinase. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling and lysosomal degradation (1,2). Phosphorylation of Tyr845 in the kinase domain may stabilize the activation loop, maintain the enzyme in an active state and provide a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of Tyr845 (5). Phospho-tyrosine 992 is a direct binding site for the phospholipase C-g (PLC-g) SH2 domain, resulting in activation of PLC-g-mediated downstreamsignaling (6). Phosphorylation of Tyr1045 creates a major docking site for c-Cbl (7). Binding of c-Cbl to the activated EGF receptor leads to receptor ubiquitination and degradation (8). Phospho-tyrosine 1068 of activated EGF receptor is a direct binding site for Grb2 (9). Phospho-tyrosine 1148 and 1173 provides a docking site for SHC (2). Both sites are involved in activation of MAP kinase signaling. Phosphorylation of EGFR on serine and threonine residues attenuates EGFR kinase activity. Ser1046/1047 in the carboxy-terminal region of EGFR are sites phosphorylated by CaM kinase II. Mutations of Ser1046/1047 upregulate tyrosine autokinase activity of EGFR (10). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:250, IHC Protocol: EDTA/TBST Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Hu Mo Rt
免疫原 :
Synthetic phosphopeptide corresponding to residues surrounding Tyr1173 of human EGF receptor.
特异性 :
Recognizes endogenous human EGF receptor only when phosphorylated at tyrosine 1173. May weakly crossreact with insulin receptor. Species Crossreactivity: mouse and rat.
纯度 :
纯化
形式 :
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Mab Isotype: IgG Clone No: 4i5(53A5) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr1173 of human EGF receptor. Specificity: Recognizes endogenous human EGF receptor only when phosphorylated at tyrosine 1173. May weakly crossreact with insulin receptor. Species Crossreactivity: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
免疫组化免疫沉淀免疫印迹
储存温度 :
-20°C
参考文献 :
1. Hackel, P. O. et al. (1999) Curr. Opin. Cell Biol. 11:184–189. 2. Zwick, E. et al. (1999) Trends Pharmacol. Sci. 20:408–412. 3. Cooper, J.A. and Howell, B. (1993) Cell 73:1051–1054. 4. Hubbard, S. R. et al. (1994) Nature 372:746–754. 5. Biscardi, J. S. et al. (1999) J. Biol. Chem. 274:8335–8343. 6. Emlet, D. R. et al. (1997) J. Biol. Chem. 272:4079–4086. 7. Levkowitz, G. et al. (1999) Mol. Cell 4:1029–1040. 8. Ettenberg, S. A. et al. (1999) Oncogene 18:1855–1866. 9. Rojas, M. et al. (1996) J. Biol. Chem. 271:27456–27461. 10. Feinmesser, R. L. et al., (1999) J. Biol. Chem. 274:16168–16173. 11. Spieker-Polet H, et al., (1995) Proc Natl Acad Sci. Sep 26;92(20):9348-52. 12. Liguori MJ, et al. (2001) Hybridoma. Jun; 20(3):189-98. 13. G.Cano, et al., (2003) Diagn Cytopathol. Oct; 29(4):207 -11. 14. L.K. Diaz and N.Sneige, (2005) Adv Anat Pathol. 12(1):10-19. 15. Z. Huang, et al., (2005) Appl Immunohistochem Mol Morphol. 13(1):91-95. 16. S. Rossi, et al., (2004) Abstract presented at USCAP 2004. Modern Pathology 17(suppl 1):361A. 17. M. Blechner, et al., (2004) Abstract presented at USCAP 2004. Modern Pathology 17(suppl 1):241A. 18. W. Cheuk, et al., (2004) Am J Surg Path. 28(6):801-807. 19. G.B. Budd, et al., (2004) Abstract presented at ASCO Annual meeting, June, New Orleans. 20. S. M. Tarr, et al., (2004) Abstract presented at Association for Molecular Pathology meeting, Los Angeles. 21. A.M. Gown, et al., (2005) Abstract presented at USCAP 2005. Modern Pathology 18(suppl.1):35A. 22. D.O. Treaba, et al., (2005) Abstract presented at USCAP 2005. Modern Pathology 18(suppl.1):53A. 23. S. Rossi, et al., (2005) Am J Clin Pathol. Aug;124(2):295-302.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/24/E3375-55_1.jpg
Picture4文本 :
Western blot analysis of extracts from BxPc-3 cells treated with EGF (100 ng/ml for 5 min) or EGF plus the EGF receptor tyrosine kinase inhibitor Gefitinib (10 uM for 2 hrs), using E3375-55 (upper) or EGF Receptor Antibody (lower).
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。