产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
ErbB2, phosphorylated (Tyr877) (c-erbB2, HER2, neu Oncoprotein)
目录 :
E3451-26B
规格 :
100微升
价格 :
566美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
E3451-26B
Product wo Prefix :
ErbB2, phosphorylated (Tyr877) (c-erbB2, HER2, neu Oncoprotein)
宿主 :
产品类型 :
多克隆抗体
抗原修饰 :
磷酸化
类别 :
抗体
尺寸1 :
100微升
Size1 List :
566美元
抗体亚型 :
IgG
Desc1 :
The ErbB2 (HER2) proto-oncogene encodes a transmembrane receptor-like glycoprotein of 185kD with intrinsic tyrosine kinase activity (1). ErbB2 does not have any known ligand; however, the kinase activity of ErbB2 can be activated without any ligand if it is overexpressed and by heteroassociation with other members of the ErbB family (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for breast cancer and other carcinomas. The therapeutic potential may result from directing ErbB2 to a c-Cblregulated proteolytic pathway. Tyrosine 877 of ErbB2 is homologous to pp60c-Src Tyr416 located in the kinase domain. Phosphorylation of this site may be involved in regulation of ErbB2 biological activity. Tyrosine 1248 of ErbB2 is one of the major autophosphorylation sites in this molecule. The phosphorylation of this site couples ErbB2 to the Ras- Raf-MAP kinase signal transduction pathway (5). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Hu
免疫原 :
Synthetic phosphopeptide corresponding to residues surrounding Tyr877 of human ErbB2.
特异性 :
Recognizes endogenous levels of human ErbB2 only when phosphorylated at tyrosine 877 at ~185kD. May crossreact with other ErbB family members (e.g., EGF receptor) when their homologous tyrosines are phosphorylated, but it does not crossreact with other tyrosine-phosphorylated proteins. Species sequence homology: mouse and rat.
纯度 :
Purified by Protein A and peptide affinity chromatography.
形式 :
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
浓度 :
未确定
Desc2 :
Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr877 of human ErbB2. Specificity: Recognizes endogenous levels of human ErbB2 only when phosphorylated at tyrosine 877 at ~185kD. May crossreact with other ErbB family members (e.g., EGF receptor) when their homologous tyrosines are phosphorylated, but it does not crossreact with other tyrosine-phosphorylated proteins. Species sequence homology: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E免疫印迹
储存温度 :
-20°C
参考文献 :
1. Muthuswamy, S.K. et al. (1999) Mol. Cell. Biol. 19:6845–6857. 2. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91:1500–1504. 3. Dittadi, R. and Gion, M. (2000) J. Natl. Cancer Inst. 92:1443–1444. 4. Klapper, L.N. et al. (2000) Cancer Res. 60:3384–3388. 5. Kwon, Y.K. et al. (1997) J. Neurosci. 17:8293–8299.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/30/E3451-26B_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。