抗体

β-Galactosidase

G1041-02

100微升

516美元

单克隆

小鼠

未共轭

3A11(14B7)

β-Galactosidase

小鼠

单克隆抗体

抗体

100微升

516美元

3A11(14B7)

IgG1

Proteins can be tagged using recombinant DNA techniques to allow for their purification and or detection in different cell systems. b-galactosidase has been used extensively as a fusion partner since b-gal fusion proteins can also be detected by quantitation b-galactosidase enzymatic activity. Epitope tags are useful for the labeling and detection of proteins using Western Blot, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. Apparent Molecular Weight: The apparent molecular weight is related to the fusion protein. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence/Immunocytochemistry Immunoprecipitation and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (IC): 1:3200 Flow Cytometry: 1:7000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

重组β半乳糖苷酶蛋白

Recognizes beta-Galactosidase. Detects beta-galactosidase fusion proteins overexpressed in cells. Does not recognize any other overexpressed protein. Species Crossreactivity: A broad species crossreactivity is expected based on conservation of sequence homology.

腹水

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

未确定

Product Type: Mab Isotype: IgG1 Clone No: 3A11(14B7) Host: mouse Concentration: Not Determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Recombinant beta galactosidase protein. Specificity: Recognizes beta-Galactosidase. Detects beta-galactosidase fusion proteins overexpressed in cells. Does not recognize any other overexpressed protein. Species Crossreactivity: A broad species crossreactivity is expected based on conservation of sequence homology. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC细胞化学IF免疫沉淀免疫印迹

-20°C

1. Draber, P. et al. (1992) Hybridoma 11, 385-390. 2. Hayashibe, K. et al. (1990) J. Immunoassay 11, 89-95. 3. Shapiro, S. and Kimmel, B. (1987) J. Immunol. Methods 97, 275-279.

https://usbio-images.r.worldssl.net/prodimages/22/G1041-02_1.jpg

Flow cytometric analysis of COS cells, untransfected (blue) or transfected with beta-galactosidase (green), using G1041-02.