抗体

Gelsolin (GSN, Actin Depolymerizing Factor, ADF, AGEL, Amyloidosis Finnish Type, Brevin, DKFZp313L0718)

G2024-53K1

100微克

532美元

多克隆

domestic goat

未共轭

人类, 小鼠, 大鼠

Gelsolin (GSN, Actin Depolymerizing Factor, ADF, AGEL, Amyloidosis Finnish Type, Brevin, DKFZp313L0718)

山羊

多克隆抗体

抗体

100微克

532美元

IgG

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity. Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:128,000 Western Blot: 0.1-0.3ug/ml, observed in human spleen lysates on ~90kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Ca Hu Mo Rt

Synthetic peptide corresponding to C-PRLKDKKMDAHP, from human GSN, at the internal region of the protein (NP_000168.1, NP_937895.1).

Recognizes human GSN. Species sequence homology: Canine, mouse and rat.

经免疫亲和层析纯化

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

~0.5毫克/毫升

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-PRLKDKKMDAHP, from human GSN, at the internal region of the protein (NP_000168.1, NP_937895.1). Specificity: Recognizes human GSN. Species sequence homology: Canine, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E免疫印迹

-20°C

1. Tanaka H, Shirkoohi R, Nakagawa K, Qiao H, Fujita H, Okada F, Hamada J, Kuzumaki S, Takimoto M, Kuzumaki N. siRNA gelsolin knockdown induces epithelial-mesenchymal transition with a cadherin switch in human mammary epithelial cells. Int J Cancer. 2006 Apr 1;118(7):1680-91.

https://usbio-images.r.worldssl.net/prodimages/39/G2024-53K1_1.jpg