抗体

糖原合酶激酶3甲, 乙(GSK3a/b), 磷酸化(Ser21/9), GSK-3a-preferred

G8170-41K

100微升

571美元

单克隆

domestic rabbit

未共轭

磷酸化

7H24(37F11)

糖原合酶激酶3甲, 乙(GSK3a/b), 磷酸化(Ser21/9), GSK-3a-preferred

兔

单克隆抗体

磷酸化

抗体

100微升

571美元

7H24(37F11)

IgG

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3a and Ser9 of GSK-3b (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium, and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilution: Immunoprecipitation: 1:50 Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic phosphopeptide corresponding to residues around Ser21 of human GSK-3α.

Recognizes endogenous levels of human GSK-3a at 51kD when phosphorylated at Ser21 and human GSK-3b at 46kD when phosphorylated at Ser9. Species Crossreactivity: monkey, mouse and rat

纯化

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

未确定

Product Type: Mab Isotype: IgG Clone No: 7H24(37F11) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues around Ser21 of human GSK-3α. Specificity: Recognizes endogenous levels of human GSK-3a at 51kD when phosphorylated at Ser21 and human GSK-3b at 46kD when phosphorylated at Ser9. Species Crossreactivity: monkey, mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫沉淀免疫印迹

-20°C

1. Welsh, G.I. et al. (1996) Trends Cell Biol. 6, 274-279. 2. Srivastava, A.K. and Pandey, S.K. (1998) Mol. Cell. Biochem. 182, 135-141. 3. Cross, D.A. et al. (1995) Nature 378, 785-789. 4. Nusse, R. (1997) Cell 89, 321-323. 5. Diehl, J.A. et al. (1998) Genes Dev. 12, 3499-3511.

https://usbio-images.r.worldssl.net/prodimages/41/G8170-41K_1.jpg