产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
HAND2 (dHAND, Thing-2, Hed, HAND Family Transcription Factor)
目录 :
H1779-70
规格 :
100微克
价格 :
735美元
克隆性 :
多克隆
宿主 :
domestic goat
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
更多信息或购买 :
产品信息
目录号 :
H1779-70
Product wo Prefix :
HAND2 (dHAND, Thing-2, Hed, HAND Family Transcription Factor)
宿主 :
山羊
产品类型 :
多克隆抗体
类别 :
抗体
尺寸1 :
100微克
Size1 List :
735美元
抗体亚型 :
IgG
Desc1 :
HAND2 (also known as dHAND, Thing-2 and Hed) is a 24kD, class B member of the basic helix-loop-helix transcription factors superfamily. HAND2 is essential for cardiac morphogenesis and required for vascular development and regulation of angiogenesis, possibly through VEGF signaling. HAND2 is also implicated in limb development acting as an upstream regulator of sonic hedgehog. Alternative initiation and internal deletions result in at least three potential HAND2 isoforms. Human and mouse HAND2 proteins are 100% identical. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml. The detection limit for rhHAND2 and rmHAND2 is approximately 1.3ng/well. Western Blot: 0.1-0.2ug/ml. The detection limit for rhHAND2 and rmHAND2 is approximately 25ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 10ug/ml to detect HAND2 in mouse embryos. Cells were fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA. After blocking, cells were incubated with diluted primary antibody followed by NorthernLightsTM 557-coupled anti-goat IgG at RT in the dark. Between each step, cells were washed with PBS containing BSA. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile PBS. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Hu Mo
免疫原 :
Recombinant protein corresponding to Met1-Gln217 of human HAND2, expressed in E. coli.
特异性 :
Recognizes human HAND2. Species Crossreactivity: mouse
纯度 :
经免疫亲和层析纯化
形式 :
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
浓度 :
~0.2mg/ml (after reconstitution)
Desc2 :
Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography. Immunogen: Recombinant protein corresponding to Met1-Gln217 of human HAND2, expressed in E. coli. Specificity: Recognizes human HAND2. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E免疫组化免疫印迹
储存温度 :
-20°C
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。