抗体

热休克蛋白27, 磷酸化(Ser82)(HSP27)

H1830-50M

20微升

355美元

多克隆

domestic rabbit

未共轭

磷酸化

热休克蛋白27, 磷酸化(Ser82)(HSP27)

兔

多克隆抗体

磷酸化

抗体

20微升

355美元

IgG

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:100 Immunohistochemistry (Frozen): 1:100 Immunofluorescence (IF-IC): 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Ser82 of human HSP27. Uniprot Accession: P04792

Detects endogenous human HSP27 only when phosphorylated at Ser82. The antibody does not recognize other heat shock proteins. Species Crossreactivity: mouse, rat and monkey

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser82 of human HSP27. Uniprot Accession: P04792 Specificity: Detects endogenous human HSP27 only when phosphorylated at Ser82. The antibody does not recognize other heat shock proteins. Species Crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF免疫组化免疫印迹

-20°C

(1) Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335–373. (2) Landry, J. et al. (1992) J. Biol. Chem. 267, 794–803. (3) Rouse, J. et al. (1994) Cell 78, 1027–1037. (4) Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947–18956. (5) Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210–24214. (6) Rousseau, S. et al. (1997) Oncogene 15, 2169–2177. Immunohistochemical analysis of paraffin-embedded human melanoma using Phospho-HSP27 (Ser82) Antibody II.

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Immunohistochemical analysis of frozen H1650 xenograft using H1830-50M

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Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using H1830-50M

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Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using H1830-50M (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).