抗体

组蛋白H3, 磷酸化(Thr11)

H5110-11R3

100微升

566美元

单克隆

domestic rabbit

未共轭

磷酸化

C2A6

组蛋白H3, 磷酸化(Thr11)

兔

单克隆抗体

磷酸化

抗体

100微升

566美元

C2A6

IgG

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23 (2,3). Acetylation at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11). Applications: Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:25 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Thr11 of human histone H3. Species sequence homology: xenopus (100%)

Detects endogenous levels of histone H3 only when phosphorylated at Thr11. This antibody does not cross-react with other phosphorylated histones. Species Crossreactivity: mouse and rat

纯化

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

未确定

Product Type: Mab Isotype: IgG Clone No: C2A6 Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr11 of human histone H3. Species sequence homology: xenopus (100%) Specificity: Detects endogenous levels of histone H3 only when phosphorylated at Thr11. This antibody does not cross-react with other phosphorylated histones. Species Crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC免疫沉淀免疫印迹

-20°C

(1) Workman, J.L. and Kingston, R.E. (1998) Annu. Rev. Biochem. 67, 545–579. (2) Hansen, J.C. et al. (1998) Biochemistry 37, 17637–17641. (3) Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41–45. (4) Cheung, P. et al. (2000) Cell 103, 263–271. (5) Bernstein, B.E. and Schreiber, S.L. (2002) Chem. Biol. 9, 1167–1173. 6) Jaskelioff, M. and Peterson, C.L. (2003) Nat. Cell Biol. 5, 395–399. (7) Thorne, A.W. et al. (1990) Eur. J. Biochem. 193, 701–713. (8) Hendzel, M.J. et al. (1997) Chromosoma 106, 348–360. (9) Goto, H. et al. (1999) J. Biol. Chem. 274, 25543–25549. (10) Preuss, U. et al. (2003) Nucleic Acids Res. 31, 878–885. (11) Dai, J. et al. (2005) Genes Dev. 19, 472–488.

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