产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
组蛋白H3
目录 :
H5110-12M8
规格 :
100微升
价格 :
555美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
10E29(D1H2)
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
H5110-12M8
Product wo Prefix :
组蛋白H3
宿主 :
产品类型 :
单克隆抗体
类别 :
抗体
尺寸1 :
100微升
Size1 List :
555美元
克隆#USB :
10E29(D1H2)
抗体亚型 :
IgG
Desc1 :
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11). Applications: Suitable for use in Western Blot, Immunohistochemistry (P) and Immunofluorescence (IF-IC). Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (P): 1:400 Unmasking buffer: Citrate Immunofluorescence (IF-IC): 1:200 IF Protocol: Methanol Permeabilization required. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Hu Mk Mo Rt
免疫原 :
Synthetic peptide corresponding to the C-terminus of the human histone H3 protein. Species Sequence Homology: bovine, melanogaster, xenopus and zebrafish.
特异性 :
Recognizes endogenous levels of total histone H3. Species crossreactivity: mouse, rat and monkey.
纯度 :
纯化
形式 :
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, ≤0.02% sodium azide, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Mab Isotype: IgG Clone No: 10E29(D1H2) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, ≤0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to the C-terminus of the human histone H3 protein. Species Sequence Homology: bovine, melanogaster, xenopus and zebrafish. Specificity: Recognizes endogenous levels of total histone H3. Species crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
细胞化学IF免疫印迹
储存温度 :
-20°C
参考文献 :
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79, 2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41, 3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5, 4. Cheung, P. et al. (2000) Cell 103, 263-71, 5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73, 6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9, 7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13, 8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60, 9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9, 10.Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85, 11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/27/H5110-12M8_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。