产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Interferon Regulatory Factor 4 (IRF4, Lymphocyte-specific interferon regulatory factor, NF-EM5, Multiple myeloma oncogene 1)
目录 :
I7662-71J
规格 :
100微升
价格 :
516美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
10E34(D43H10)
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
I7662-71J
Product wo Prefix :
Interferon Regulatory Factor 4 (IRF4, Lymphocyte-specific interferon regulatory factor, NF-EM5, Multiple myeloma oncogene 1)
宿主 :
产品类型 :
单克隆抗体
类别 :
抗体
尺寸1 :
100微升
Size1 List :
516美元
克隆#USB :
10E34(D43H10)
抗体亚型 :
IgG
Desc1 :
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-induc-ible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3gamma/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7 and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA binding domains. IRF family members regulate transcription through interac-tions with proteins that share similar DNA binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS) and IFN regulatory elements (IRF-E) (2). IRF-4 was independently cloned by three groups and demonstrated to have roles in different contexts of lymphoid regulation (3-5). First, IRF-4 (Pip) was found to associate with PU.1, a hematopoietic specific member of the ETS fam-ily, and to regulate the expression of B-cell specific genes (3). Second, it was characterized as a lymphoid-specific member of the IRF family (LSIRF) able to bind to ISRE (4). Third, it was identified in activated T cells as a factor that binds to the promoter of the interleukin-5 gene (ICSAT), and shown to repress gene activation induced by IFN (5). IRF-4 is expressed in all stages of B cell development and in mature T cells, and is inducible in primary lymphocytes by antigen mimetic stimuli such as concavalin A, CD3 cross-linking, anti-IgM and PMA treatment (4,5). Mice deficient in IRF-4 show normal distribution of B and T lymphocytes at 4 to 5 weeks, but later develop progressive generalized lymphadenopathy, suggesting a role for IRF-4 in the function and homeostasis of mature B- and T-lymphocytes (6). Applications: Suitable for use in ChIP, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 ChIP: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu Rt
免疫原 :
Synthetic peptide corresponding to residues around Asp175 of human IRF-4.
特异性 :
Recognizes endogenous levels of total human IRF-4 protein. Both alternatively spliced isoforms are detected. Does not cross-react with other family members at physiological levels. Species crossreactivity: rat.
纯度 :
纯化
形式 :
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Mab Isotype: IgG Clone No: 10E34(D43H10) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues around Asp175 of human IRF-4. Specificity: Recognizes endogenous levels of total human IRF-4 protein. Both alternatively spliced isoforms are detected. Does not cross-react with other family members at physiological levels. Species crossreactivity: rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
ChIP免疫沉淀免疫印迹
储存温度 :
-20°C
参考文献 :
1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55, 2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58, 3. Eisenbeis, C.F. et al. (1995) Genes Dev 9, 1377-87, 4. Matsuyama, T. et al. (1995) Nucleic Acids Res 23, 2127-36, 5. Yamagata, T. et al. (1996) Mol Cell Biol 16, 1283-94, 6. Mittrücker, H.W. et al. (1997) Science 275, 540-3.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/24/I7662-71J_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。