Product wo Prefix :
Size1 List :
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). IRF-5 is expressed in lymphoid tissues and peripheral blood lymphocytes and participates in the induction of type I interferon genes following viral infection (3). Activation of IRF-5 signaling is triggered by components of the toll-like receptor (TLR) pathway, including TLR7 and MyD88 (4,5). Genetic variants of IRF-5 have been associated with disorders where the IFN pathway is abnormally activated, such as systemic lupus erythematosus (6,7). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Synthetic peptide corresponding to residues surrounding Ser172 of mouse Interferon Regulatory Factor 5 (Q13568).
Recognizes endogenous levels of total mouse Interferon Regulatory Factor 5 at 60kD. Species Crossreactivity: rat
Purified by Protein A and peptide affinity chromatography.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Product Type: Pab Isotype: IgG Host: rabbit Source: mouse Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Ser172 of mouse Interferon Regulatory Factor 5 (Q13568). Specificity: Recognizes endogenous levels of total mouse Interferon Regulatory Factor 5 at 60kD. Species Crossreactivity: rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
Taniguchi, T. et al. (2001) Annu. Rev. Immunol. 19, 623-655.Honda, K. and Taniguchi, T. (2006) Nat. Rev. Immunol. 6, 644-658.Barnes, B.J. et al. (2001) J. Biol. Chem. 276, 23382-23390.Takaoka, A. et al. (2005) Nature 434, 243-249.Schoenemeyer, A. et al. (2005) J. Biol. Chem. 280, 17005-17012.Sigurdsson, S. et al. (2005) Am. J. Hum. Genet. 76, 528-537.Graham, G. et al. (2006) Nat. Genet. 38, 550-555.
Picture 1 File Name :
4 Technology Way
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