抗体

IP3 Receptor discontinued

I8471-01

100微升

438美元

多克隆

domestic rabbit

未共轭

IP3 Receptor discontinued

兔

多克隆抗体

抗体

100微升

438美元

IgG

Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family, and is located in the endo- plasmic reticulum. It functions as a Ca2+ release channel for intracellular stores of calcium ions. There are three types of IP3 receptors (IP3 receptor 1, 2 and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation (1). Four individual receptor subunits, resulting in homo- or hetero-oligomerization of the receptor isoform, form a functional channel (2). Phosphorylation of IP3R1 at Ser1756 by cyclic-AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may thus be a mode of regulation for Ca2+ release (3,4). IP3R1 mediated calcium release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells (5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

Synthetic peptide surrounding Met2750 of human IP3 receptor.

Recognizes endogenous levels of total human IP3 receptor type 1 protein at ~320kD. SpecIes Crossreactivity: mouse, rat

Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

未确定

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic peptide surrounding Met2750 of human IP3 receptor. Specificity: Recognizes endogenous levels of total human IP3 receptor type 1 protein at ~320kD. SpecIes Crossreactivity: mouse, rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫沉淀免疫印迹

-20°C

(1) Joseph, S.K. (1996) Cell Signal 8, 1–7. (2) Galvan, D.L. et al. (1999) J Biol Chem 274, 29483–92. (3) Haug, L.S. et al. (1999) J Biol Chem 274, 7467–73. (4) DeSouza, N. et al. (2002) J Biol Chem 277, 39397–400. (5) Inoue, T. et al. (1998) J Neurosci 18, 5366–73.

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