产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Luciferase
目录 :
L5999-04
规格 :
200微克
价格 :
661美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
3i1
更多信息或购买 :
产品信息
目录号 :
L5999-04
Product wo Prefix :
Luciferase
宿主 :
小鼠
产品类型 :
单克隆抗体
类别 :
抗体
尺寸1 :
200微克
Size1 List :
661美元
克隆#USB :
3i1
抗体亚型 :
IgG1
Desc1 :
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 0.5-2ug/ml detects 5ng of recombinant luciferase that was added to 3T3 cell lysate. 3T3 cell lysate containing 5ng of recombinant luciferase was resolved by electrophoresis, transferred to nitrocellulose and probed with L5999-04. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunohistochemistry: This antibody has been used by an independent laboratory for qualitative and quantitative assessment of gene expression systems employing luciferase reporter constructs in Drosophila embryos.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
免疫原 :
Full-length recombinant luciferase from Photinus pyralis.
特异性 :
Recognizes luciferase, Mr 66kD. Species Crossreactivity: P. pyralis.
纯度 :
Purified by Protein G affinity chromatography.
形式 :
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
浓度 :
~0.5毫克/毫升
Desc2 :
Product Type: Mab
Isotype: IgG1
Clone No: 3i1
Host: mouse
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Purity: Purified by Protein G affinity chromatography.
Immunogen: Full-length recombinant luciferase from Photinus pyralis.
Specificity: Recognizes luciferase, Mr 66kD. Species Crossreactivity: P. pyralis.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
免疫组化免疫印迹
储存温度 :
-20°C
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/38/L5999-04_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
tech@usbio.net
https://www.usbio.net800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。
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