抗体

Luciferase (Photinus pyralis, Firefly)

L5999-05

2毫升

762美元

多克隆

domestic goat

未共轭

Luciferase (Photinus pyralis, Firefly)

山羊

多克隆抗体

抗体

2毫升

762美元

IgG

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. Applications: Suitable for ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 to 1:100,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Firefly Luciferase (Photinus pyralis)

Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Luciferase (Firefly).

Purified by delipidation and defibrination.

以溶解在PBS液体方式提供, pH值7.2, 0.01% 叠氮化钠, 40% 甘油

~95毫克/毫升(总数蛋白)

Product Type: Pab Isotype: IgG Host: goat Concentration: ~95mg/ml (Total Protein) Form: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glycerol. Purity: Purified by delipidation and defibrination. Immunogen: Firefly Luciferase (Photinus pyralis) Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Luciferase (Firefly). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E免疫印迹

4°C(-20°C甘油)