抗体

5'-Nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT)

N7100-71B

100微克

758美元

多克隆

家羊

未共轭

5'-Nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT)

羊

多克隆抗体

抗体

100微克

758美元

IgG

CD73 (Ecto-5'nucleotidase, 5′'-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells. Applications: Suitable for use in Direct ELISA, Western Blot. and Flow Cytometry. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml. Detection limit for rmCD73 is ~0.3ng/well. Western Blot: 0.1-0.2ug/ml. Detection limit for rmCD73 is ~1ng/lane under non-reducing and reducing conditions. Flow Cytometry (Murine splenocytes): 25ug/ml. Add 100ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not excedding 200ul. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile PBS. Reconstituted product is stable for 12 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Mo

Recombinant protein corresponding to Trp29-Lys549 from mouse 5'‑Nucleotidase/CD73, expressed in CHO cells.

识别小鼠CD73

经免疫亲和层析纯化

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

~0.2mg/ml (after reconstitution)

Product Type: Pab Isotype: IgG Host: sheep Source: mouse Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography. Immunogen: Recombinant protein corresponding to Trp29-Lys549 from mouse 5'‑Nucleotidase/CD73, expressed in CHO cells. Specificity: Recognizes mouse CD73. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC免疫印迹

-20°C