产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
p53, 磷酸化(Thr81)
目录 :
P1001-25F
规格 :
100微升
价格 :
566美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
P1001-25F
Product wo Prefix :
p53, 磷酸化(Thr81)
宿主 :
产品类型 :
多克隆抗体
抗原修饰 :
磷酸化
类别 :
抗体
尺寸1 :
100微升
Size1 List :
566美元
抗体亚型 :
IgG
Desc1 :
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to reduced interaction of p53 with its negative regulator, oncoprotein MDM2 (4). MDM2 inhibits the accumulation of p53 by targeting it for ubiquitination and proteasomal degradation (6,7). p53 can apparently be phosphorylated by ATM, ATR and DNA-PK at Ser15 and Ser37; the phosphorylations impair the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,5). Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity (8,9). Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry: (Paraffin) 1:600 Citrate/TBST Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Hu Mk
免疫原 :
Synthetic phosphopeptide corresponding to residues surrounding Thr81 of human p53.
特异性 :
Detects endogenous levels of p53 only when phosphorylated at threonine 81. Species Crossreactivity: Human and monkey
纯度 :
Purified by Protein A and peptide affinity chromatography.
形式 :
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr81 of human p53. Specificity: Detects endogenous levels of p53 only when phosphorylated at threonine 81. Species Crossreactivity: Human and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
免疫组化免疫印迹
储存温度 :
-20°C
参考文献 :
(1) Levine, A.J. (1997) Cell 88, 323–331. (2) Meek, D.W. (1994) Semin. Cancer Biol. 5, 203–210. (3) Milczarek, G.J. et al. (1997) Life Sci. 60, 1–11. (4) Shieh, S.Y. et al. (1997) Cell 91, 325–334. (5) Tibbetts, R.S. et al. (1999) Genes Dev. 13, 152–157. (6) Chehab, N.H. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 13777–13782. (7) Honda, R. et al. (1997) FEBS Lett. 420, 25–27. (8) Shieh, S.Y. et al. (1999) EMBO J. 18, 1815–1823. (9) Hirao, A. et al. (2000) Science 287, 1824–1827. (10) Kohn, K.W. (1999) Mol. Biol. Cell 10, 2703–2734. (11) Hao, M. et al. (1996) J. Biol. Chem. 271, 29380–29385. (12) Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923–5934. (13) Lohrum, M. and Scheidtmann, K.H. (1996) Oncogene 13, 2527–2539. (14) Ulrich, S.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 5954–5958. (15) Knippschild, U. et al. (1997) Oncogene 15, 1727–1736. (16) Oda, K. et al. (2000) Cell 102, 849–862. (17) Blaydes, J.P. et al. (2001) J. Biol. Chem. 276, 4699–4708. (18) Katayama, H. et al. (2004) Nat. Genet. 36, 55–63.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/33/P1001-25F_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。