抗体

Paxillin (PXN)

P3113-90H

100微升

516美元

多克隆

domestic rabbit

未共轭

Paxillin (PXN)

兔

多克隆抗体

抗体

100微升

516美元

IgG

Paxillin is a multi-domain protein that localizes primarily in focal adhesion sites to the extracellular matrix (1). Paxillin is one of the key components of integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at tyrosine 118 (3,4). Phospho-Tyr118 may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5). Applications: Suitable for use in Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic peptide (KLH coupled) corresponding to residues near the carboxy- terminal sequence of human paxillin

Detects endogenous levels of paxillin from various cell lines. Does not crossreact with other related proteins

蛋白质A和肽亲和色谱

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol

未确定

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol Purity: Protein A and peptide affinity chromatography Immunogen: Synthetic peptide (KLH coupled) corresponding to residues near the carboxy- terminal sequence of human paxillin Specificity: Detects endogenous levels of paxillin from various cell lines. Does not crossreact with other related proteins Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫印迹

-20°C

(1) Turner, C.E. (2000) J. Cell Sci. 113, 4139–4140. (2) Burridge, K. et al. (1992) J. Cell Biol. 119, 893–903. (3) Bellis, S.L. et al. (1995) J. Biol. Chem. 270, 17437–17441. (4) Bellis, S.L. et al. (1997) Biochem. J. 325, 375–381. (5) Schaller, M.D. and Parsons, J.T. (1995) Mol. Cell. Biol. 15, 2635–2645.

https://usbio-images.r.worldssl.net/prodimages/31/P3113-90H_1.jpg