抗体

PDI(蛋白质二硫键异构酶 )

P3122-11

100微升

516美元

多克隆

domestic rabbit

未共轭

PDI(蛋白质二硫键异构酶 )

兔

多克隆抗体

抗体

100微升

516美元

IgG

Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds. This process is called oxidative protein folding. Disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds. Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange. This event is then followed by PDI-catalyzed disulfide bond formation on folding proteins. Applications: Suitable for use in Western Blot, Immunofluorescence/ Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:100. Antigen retrieval in citrate buffer required. Immunofluorescence (IC): 1:50; Requires methanol permeabilization. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide corresponding to residues surrounding Pro329 of human PDI.

Recognizes endogenous levels of total human PDI protein at ~57kD. Species Crossreactivity: monkey, mouse and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

未确定

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Pro329 of human PDI. Specificity: Recognizes endogenous levels of total human PDI protein at ~57kD. Species Crossreactivity: monkey, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

细胞化学IF免疫组化免疫印迹

-20°C

1. Huppa, J.B. & Ploegh, H.L., Cell 92: 145-148 (1998). 2. Ellgaard, L. & Ruddock, L.W., EMBO Rep. 6: 28-32 (2005). 3. Tu, B.P. & Weissman, J.S., J. Cell Biol. 164: 341-346 (2004).

https://usbio-images.r.worldssl.net/prodimages/34/P3122-11_1.jpg

Confocal immunofluorescent analysis of C2C12 cells using P3122-11 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).