抗体

磷脂酶C, 丙1(PLCg1)

P4074-42N

100微升

516美元

多克隆

domestic rabbit

未共轭

磷脂酶C, 丙1(PLCg1)

兔

多克隆抗体

抗体

100微升

516美元

IgG

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli, such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5- triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLC5, PLCbeta, PLCgamma, PLCdelta and PLCepsilon have been identified so far. The PLCbeta subfamily includes four members, PLCbeta1–4. All four members of the subfamily are activated by alpha-or beta-gamma-subunits of the heterotrimeric G-proteins (2,3). Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of serine 1105 by PKA or PKC inhibits PLCbeta3 activity (4,5). Serine 537 of PLCbeta3 is phosphorylated by CaMKII and this phosphorylation may contribute to the basal activity of PLCbeta3. PLCgamma is activated by both receptor and nonreceptor tyrosine kinases (6). PLCgamma forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCgamma1 on tyrosine residues 771, 783 and 1245 (7). Phosphorylation by Syk at tyrosine 783 activates the enzymatic activity of PLCgamma1 (8). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Rt

Synthetic peptide corresponding to the C-terminal residues of human Phospholipase C, gamma 1 (PLCg1).

Recognizes endogenous levels of total human PLC gamma1 protein at ~155kD. Does not crossreact with PLCgamma2. Species Crossreactivity: mouse and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

未确定

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the C-terminal residues of human Phospholipase C, gamma 1 (PLCg1). Specificity: Recognizes endogenous levels of total human PLC gamma1 protein at ~155kD. Does not crossreact with PLCgamma2. Species Crossreactivity: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫沉淀免疫印迹

-20°C

1. Singer, W.D. et al. (1997) Annu. Rev. Biochem. 66, 475–509. 2. Smrcka, A.V. et al. (1991) Science 251, 804–807. 3. Taylor, S.J. et al. (1991) Nature 350, 516–518. 4. Yue, C. et al. (1998) J. Biol. Chem. 273, 18023–18027. 5. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220–30225. 6. Margoris, B. et al. (1989) Cell 57, 1101–1107. 7. Kim, H.K. et al. (1991) Cell 65, 435–442. 8. Wang, Z. et al. (1998) Mol. Cell. Biol. 18, 590–597.

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