磷脂酶C, 丙1, 磷酸化(Tyr783)(PLCg1)
Product wo Prefix :
磷脂酶C, 丙1, 磷酸化(Tyr783)(PLCg1)
Size1 List :
Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli, such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5- triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCbeta, PLCgamma, PLCdelta and PLCepsilon. The PLCbeta subfamily includes four members, PLCbeta1–4. All four members of the subfamily are activated by alpha-or beta-gamma-subunits of the heterotrimeric G-proteins (2,3). Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of serine 1105 by PKA or PKC inhibits PLCbeta3 activity (4,5). Serine 537 of PLCbeta3 is phosphorylated by CaMKII and this phosphorylation may contribute to the basal activity of PLCbeta3. PLCgamma is activated by both receptor and nonreceptor tyrosine kinases (6). PLCgamma forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCgamma1 on tyrosine residues 771, 783 and 1245 (7). Phosphorylation by Syk at tyrosine 783 activates the enzymatic activity of PLCgamma1 (8). Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Flow Cytometry: 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Hu Mo Rt
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr783 of human PLC gamma 1.
Recognizes human PLC gamma 1 only when activated by phosphorylation at Tyr783. It does not crossreact with phosphorylated PLC gamma 2 or other PLCs. Species Crossreactivity: Mouse and rat.
Purified by Protein A and peptide affinity chromatography.
Supplied as liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr783 of human PLC gamma 1. Specificity: Recognizes human PLC gamma 1 only when activated by phosphorylation at Tyr783. It does not crossreact with phosphorylated PLC gamma 2 or other PLCs. Species Crossreactivity: Mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
1. Singer, W.D., et al., Annu. Rev. Biochem. 66: 475–509 (1997). 2. Smrcka, A.V., et al., Science 251: 804–807 (1991). 3. Taylor, S.J., et al., Nature 350: 516–518 (1991). 4. Yue, C., et al., J. Biol. Chem. 273: 18,023-18,027 (1998). 5. Yue, C., et al., J. Biol. Chem. 275: 30,220-30,225 (2000). 6. Margolis, B., et al., Cell 57: 1101–1107 (1989). 7. Kim, H.K., et al., Cell 65: 435–442 (1991). 8. Wang, Z., et al., Mol. Cell. Biol. 18: 590- 597 (1998).
Picture 1 File Name :
4 Technology Way
United States Biological是生命科学领域的知名品牌，致力于用真实、有价值的个性化采购经验降低研究用成本。