产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
磷酸酪氨酸
目录 :
P4075-28
规格 :
1毫克
价格 :
1012美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
3G239
更多信息或购买 :
产品信息
目录号 :
P4075-28
Product wo Prefix :
磷酸酪氨酸
宿主 :
小鼠
产品类型 :
单克隆抗体
类别 :
抗体
尺寸1 :
1毫克
Size1 List :
1012美元
克隆#USB :
3G239
抗体亚型 :
IgG2b
Desc1 :
Specific reversible protein phosphorylation plays a central role in regulating many cellular events by controlling the activity of participating proteins. Ways that phosphorylation/dephosphorylation can modulate a protein’s activity include altering its conformation, localization, stability, and ability to associate or form complexes with other proteins(3-5). As much as one third of cellular proteins may be phosphorylated at any given moment. Reversible protein tyrosine phosphorylations are central to regulating events in signal transduction pathways initiated by most growth factors and cytokines. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunoprecipitation: 2-5ug Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
免疫原 :
KLH coupled with equal amounts of phosphotyrosine, glycine, and alanine.
特异性 :
Recognizes specifically with tyrosine phosphorylated proteins in the native or denatured states. It does not react with phosphoserine or phosphothreonine.(1) Binding of PY20 to phosphotyrosine containing proteins or peptides is inhibited by free phosphotyrosine. Species Crossreactivity: Recognition of phosphotyrosine in peptides or proteins is independent of species of origin.
纯度 :
Purified by Protein A affinity chromatography.
形式 :
Supplied as a liquid in 10mM sodium phosphate, pH 7.4, 0.05% sodium azide.
浓度 :
~0.25毫克/毫升
Desc2 :
Product Type: Mab Isotype: IgG2b Clone No: 3G239 Host: mouse Concentration: ~0.25mg/ml Form: Supplied as a liquid in 10mM sodium phosphate, pH 7.4, 0.05% sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: KLH coupled with equal amounts of phosphotyrosine, glycine, and alanine. Specificity: Recognizes specifically with tyrosine phosphorylated proteins in the native or denatured states. It does not react with phosphoserine or phosphothreonine.(1) Binding of PY20 to phosphotyrosine containing proteins or peptides is inhibited by free phosphotyrosine. Species Crossreactivity: Recognition of phosphotyrosine in peptides or proteins is independent of species of origin. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
免疫沉淀免疫印迹
储存温度 :
-20°C
参考文献 :
1. Glenney Jr., J.R., et al, J. Immunol. Methods 109:277 (1988). 2. Sargiacomo, M., et al, J. Cell Biol. (1993). 3. Hunter, T., Meth. Enzymol. 200:3–37 (1991). 4. Hunter, T., Cell 50:823–829 (1987). 5. Hunter, T., Cell 80:225–236 (1987). 6. Bourguignon, L.Y., et al., J. Biol. Chem. 272:27913–27918 (1997). 7. Jackson, D. et al, J. Biol. Chem. 272(11): 6986–6993 (1997). 8. Raffioni, S. and Bradshaw, R.A., J. Biol, Chem, 270:7568–7572 (1995). 9. Raja, S. et al, J. Biol. Chem. 272(16): 10941–10947 (1997). 10. Zrihan-Licht, S. et al. J. Biol. Chem. 272(3): 1856–1863 (1997). 11. Jackson, D. et al, J. Biol. Chem. 272(40): 24868–24875 (1997). 12. Jackson, D. et al; J. Biol. Chem. 272(11): 6986–6993 (1997). 13. Greebera. S., et al; J. Biol. Chem. 269(5):3897–3902 (1994). 14. Dash, D., et al; J. Biol. Chem. 270(29):17321–17326 (1995). 15. Lawler, S., et al; J. Biol. Chem. 272(23):14850–14859 (1997). 16. Chen, J.K., et al; J. Biol. Chem. 273(44):29254–29261 (1998).
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。