抗体

Protein Kinase C, delta (PKCd)

P9103-19L

100微升

516美元

多克隆

domestic rabbit

未共轭

Protein Kinase C, delta (PKCd)

兔

多克隆抗体

抗体

100微升

516美元

IgG

Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction. PKC isoforms have been classified into three groups: classical PKCs, which are Ca2+ dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG) and phorbolesters (TPA or PMA) through their cysteine rich C1 domains; novel PKCs, which are Ca2+ independent but are still regulated by PS, DAG and TPA; and atypical PKCs, which are Ca2+ independent and do not require PS, DAG or TPA for their activation. Members of these three PKC groups contain a pseudosubstrate or autoinhibitory domain that binds to the substrate binding site in the catalytic domain, preventing its activation in the absence of cofactors or activators. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide corresponding to human Protein Kinase C, delta.

Recognizes endogenous levels of total human PKCd at ~78kD. Does not crossreact with endogenous levels of other PKC isoforms. Species Crossreactivity: monkey, mouse, rat

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

未确定

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to human Protein Kinase C, delta. Specificity: Recognizes endogenous levels of total human PKCd at ~78kD. Does not crossreact with endogenous levels of other PKC isoforms. Species Crossreactivity: monkey, mouse, rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫印迹

-20°C

(1) Nishizuka, Y. (1984) Nature 308, 693–698. (2) Keranen, L.M. et al. (1995) Curr. Biol. 5, 1394–1403. (3) Newton, A.C. (1995) J. Biol. Chem. 270, 28495–28498. (4) Mellor, H. and Parker, P.J. (1998) Biochem. J. 332, 281–292. (5) Ron, D. and Kazanietz, M.G. (1999) FASEB J. 13, 1658–1676. (6) Way, K.J. et al. (2000) Trends Pharmacol. Sci. 21, 181–187. (7) Moscat, J. and Diaz-Meco, M.T. (2000) EMBO Rep. 1, 399–403. (8) Good, J.A. et al. (1998) Science 281, 2042–2045. (9) Sonnenburg, E.D. (2001) J. Biol. Chem. 276, 45289–45297.

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