产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Ras相关C3肉毒杆菌毒素底物1/Cdc42
目录 :
R0400-05D
规格 :
20微升
价格 :
342美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
R0400-05D
Product wo Prefix :
Ras相关C3肉毒杆菌毒素底物1/Cdc42
宿主 :
产品类型 :
多克隆抗体
类别 :
抗体
尺寸1 :
20微升
Size1 List :
342美元
抗体亚型 :
IgG
Desc1 :
Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development (1). GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu Mk Mo Rt
免疫原 :
Synthetic peptide corresponding to residues near the N-termini of human rac1 and cdc42.
特异性 :
Recognizes endogenous levels of total rac1 and cdc42 protein. Based on sequence similarities, the antibody may react with other small GTPases. Species crossreactivity: Human, mouse, rat and monkey
纯度 :
Purified by Protein A and peptide affinity chromatography.
形式 :
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues near the N-termini of human rac1 and cdc42. Specificity: Recognizes endogenous levels of total rac1 and cdc42 protein. Based on sequence similarities, the antibody may react with other small GTPases. Species crossreactivity: Human, mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
免疫印迹
储存温度 :
-20°C
参考文献 :
1. Wennerberg, K. and Der, C.J. (2004) J. Cell Sci. 117, 1301-1312. 2. Bernards, A. and Settleman, J. (2004) Trends Cell Biol 14, 377-85. 3. Rossman, K.L. et al. (2005) Nat Rev Mol Cell Biol 6, 167-80.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/8/R0400-05D_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。