产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Ras, pan, N,H,K
目录 :
R1198-01D
规格 :
100微升
价格 :
516美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
7H56(27H5)
反应物种 :
人类
应用 :
免疫印迹
更多信息或购买 :
文章摘录数: 1
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 5a
Friedrich T, Söhn M, Gutting T, Janssen K, Behrens H, Rocken C, et al. Subcellular compartmentalization of docking protein-1 contributes to progression in colorectal cancer. EBioMedicine. 2016;8:159-172 pubmed 出版商
产品信息
目录号 :
R1198-01D
Product wo Prefix :
Ras, pan, N,H,K
宿主 :
产品类型 :
单克隆抗体
类别 :
抗体
尺寸1 :
100微升
Size1 List :
516美元
克隆#USB :
7H56(27H5)
抗体亚型 :
IgG
Desc1 :
The 21kD guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Dr Hu Mk Mo Rt
免疫原 :
Synthetic peptide corresponding to residues near the amino terminus of human K-Ras.
特异性 :
Recognizes endogenous levels of human total K-Ras, H-Ras and N-Ras proteins at ~21kD. Species Crossreactivity: mouse, monkey, rat and D. melanogaster
纯度 :
纯化
形式 :
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Mab Isotype: IgG Clone No: 7H56(27H5) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues near the amino terminus of human K-Ras. Specificity: Recognizes endogenous levels of human total K-Ras, H-Ras and N-Ras proteins at ~21kD. Species Crossreactivity: mouse, monkey, rat and D. melanogaster Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E免疫印迹
储存温度 :
-20°C
参考文献 :
1. Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-654. 2. Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283. 3. Buday, L. and Downward, J. (1993) Cell 73, 611-620. 4. Huang, D.C. et al. (1993) Mol. Cell Biol. 13, 2420-2431. 5. Bos, J.L. (1989) Cancer Res. 49, 4682-4689. 6. Ma, J. and Karplus, M. (1997) J. Mol. Biol. 274, 114-131.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/45/R1198-01D_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。