Ras, pan, N,H,K
Product wo Prefix :
Ras, pan, N,H,K
Size1 List :
The 21kD guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Dr Hu Mk Mo Rt
Synthetic peptide corresponding to residues near the amino terminus of human K-Ras.
Recognizes endogenous levels of human total K-Ras, H-Ras and N-Ras proteins at ~21kD. Species Crossreactivity: mouse, monkey, rat and D. melanogaster
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Product Type: Mab Isotype: IgG Clone No: 7H56(27H5) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues near the amino terminus of human K-Ras. Specificity: Recognizes endogenous levels of human total K-Ras, H-Ras and N-Ras proteins at ~21kD. Species Crossreactivity: mouse, monkey, rat and D. melanogaster Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
1. Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-654. 2. Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283. 3. Buday, L. and Downward, J. (1993) Cell 73, 611-620. 4. Huang, D.C. et al. (1993) Mol. Cell Biol. 13, 2420-2431. 5. Bos, J.L. (1989) Cancer Res. 49, 4682-4689. 6. Ma, J. and Karplus, M. (1997) J. Mol. Biol. 274, 114-131.
Picture 1 File Name :
4 Technology Way
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