抗体

RasGRP3

R1198-10G

100微升

516美元

单克隆

domestic rabbit

未共轭

C33A3

RasGRP3

兔

单克隆抗体

抗体

100微升

516美元

C33A3

IgG

Lymphocyte activation occurs in part through activation of the Ras signaling pathway following lymphocyte receptor stimulation. The RasGRP family of guanine nucleotide exchange factors (GEFs) catalyzes the exchange of GDP for GTP on Ras family small GTPases, promoting their active GTP-bound form. Diacylglycerol (DAG) or phorbol ester binding to RasGRP family members causes their translocation to the cell membrane and stimulates their activity (1,2). While T-Cells express RasGRP1, B-cells express both RasGRP1 and RasGRP3. RasGRP3 is important in linking B-cell receptor (BCR) activation to Ras signaling (3). In response to BCR stimulation, RasGRP3 is phosphorylated at Thr133 by PKC. This phosphorylation event further activates RasGRP3 in response to DAG, which stimulates PKC activity (4,5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic peptide corresponding to residues near the C-teminus of human RasGRP3. Species sequence homology: monkey (100%)

Recognizes endogenous levels of total human RasGRP3. Species Crossreactivity: mouse

纯化

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

未确定

Product Type: Mab Isotype: IgG Clone No: C33A3 Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues near the C-teminus of human RasGRP3. Species sequence homology: monkey (100%) Specificity: Recognizes endogenous levels of total human RasGRP3. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫印迹

-20°C

(1) Braun, D.C. et al. (2005) Mol Cancer Ther 4, 141–50. (2) Lorenzo, P.S. et al. (2001) Cancer Res 61, 943–9. (3) Oh-hora, M. et al. (2003) J Exp Med 198, 1841–51. (4) Aiba, Y. et al. (2004) Proc Natl Acad Sci USA 101, 16612–7. (5) Zheng, Y. et al. (2005) Blood 105, 3648–54.

https://usbio-images.r.worldssl.net/prodimages/21/R1198-10G_1.jpg