抗体

RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kD, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34)

R3400-02Y

100微升

516美元

单克隆

大鼠

未共轭

7H60(4E4)

RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kD, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34)

大鼠

单克隆抗体

抗体

100微升

516美元

7H60(4E4)

IgG1

RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14kD subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70kD subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication, recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs. In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 checkpoint complex. RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol alpha, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted R3400-02Y in 5% w/v nonfat dry milk, 1x TBS, 0.1% Tween20 at 4ºC with gentle shaking overnight. Immunoprecipitation: 1:100 Immunofluorescence (IF-IC): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hm Hu Mk Mo Rt

Recombinant full-length protein corresponding to human MBP-RPA32 protein.

Recognizes endogenous levels at the carboxy-terminal of total human RPA32 at ~32kD. Species Crossreactivity: hamster, monkey, mouse and rat

腹水

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol

未确定

Product Type: Mab Isotype: IgG1 Clone No: 7H60(4E4) Host: rat Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol Purity: Ascites Immunogen: Recombinant full-length protein corresponding to human MBP-RPA32 protein. Specificity: Recognizes endogenous levels at the carboxy-terminal of total human RPA32 at ~32kD. Species Crossreactivity: hamster, monkey, mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

细胞化学IF免疫沉淀免疫印迹

-20°C

1. Liu, V.F. and Weaver, D.T. (1993) Mol. Cell Biol. 13:7222-7231. 2. Wobbe, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84: 1834-1838. 3. Fairman, M.P. and Stillman, B. (1988) EMBO J. 7: 1211-1218. 4. Wold, M.S. and Kelly, T. (1988) Proc. Natl. Acad. Sci.USA 85: 2523-2527. 5. Zhou, B.B. and Elledge, S.J. (2000) Nature 408: 433-439. 6. Kastan, M.B. and Bartek, J. (2004) Nature 432: 316–323. 7. Sancar, A. et al. (2004) Annu. Rev. Biochem. 73: 39-85. 8. Guo, S. et al. (2006) J. Biol. Chem. Epub ahead of print. 9. Wu, X. et al. (2005) Oncogene 24: 4728-4735. 10. Binz, S.K. et al. DNA Repair (Amst) 3: 1015-1024. 11. Nuss, J.E. et al. (2005) Biochemistry 44: 8428-8437. 12. Yuzhakov, A. et al. (1999) EMBO J. 18: 6189-6199.

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