产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Shc, phosphorylated (Tyr239/240) (SHC1, Src Homology and Collagen, Src Homology 2, p52SHC, p66SHC, SH2 Domain Protein C1, SHC, SHCA, SHC Transforming Protein 1)
目录 :
S1010-90B
规格 :
100微升
价格 :
566美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
S1010-90B
Product wo Prefix :
Shc, phosphorylated (Tyr239/240) (SHC1, Src Homology and Collagen, Src Homology 2, p52SHC, p66SHC, SH2 Domain Protein C1, SHC, SHCA, SHC Transforming Protein 1)
宿主 :
产品类型 :
多克隆抗体
抗原修饰 :
磷酸化
类别 :
抗体
尺寸1 :
100微升
Size1 List :
566美元
抗体亚型 :
IgG
Desc1 :
Shc possesses SH2 and PTB domains. It serves a scaffolding function in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms. These isoforms are produced by using alternative translation initiation sites or a differentially spliced message (1-3). In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317 (4-6). Grb2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway (4). Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development (7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Hu Mo Rt
免疫原 :
Synthetic phosphopeptide corresponding to residues surrounding Tyr239/240 of human Shc (KLH coupled).
特异性 :
Detects Shc only when phosphorylated at tyrosine 239/240. May crossreact with activiated EGF receptor protein. Species Crossreactivity: Human, mouse and rat.
纯度 :
Purified by Protein A and peptide affinity chromatography.
形式 :
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr239/240 of human Shc (KLH coupled). Specificity: Detects Shc only when phosphorylated at tyrosine 239/240. May crossreact with activiated EGF receptor protein. Species Crossreactivity: Human, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
免疫印迹
储存温度 :
-20°C
参考文献 :
1. Pelicci, G., et al., Cell 70: 93-104 (1992). 2. Migliaccio, E., et al., EMBO J. 16: 706-716 (1997). 3. Okada, S., et al., J. Biol. Chem. 272: 28042-28049 (1997). 4. Salcini, A.E., et al., Oncogene 9: 2827-2836 (1994). 5. Gotoh, N., et al., EMBO J. 15: 6197-6204 (1996). 6. Van der Geer, P., et al., Curr. Biol. 6: 1435-1444 (1996). 7. Zhang, L., et al., Nat. Immunol. 3: 749-755 (2002).
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/8/S1010-90B_1.jpg
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。