抗体

Smac (DIABLO, Direct IAP Binding Protein of Low Pi, Second Mitochondrial Activator of Cell Death)

S1014-48E

100微升

516美元

单克隆

小鼠

未共轭

4i16

Smac (DIABLO, Direct IAP Binding Protein of Low Pi, Second Mitochondrial Activator of Cell Death)

小鼠

单克隆抗体

抗体

100微升

516美元

4i16

IgG1

Smac/Diablo is a 21kD mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrialstress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3 (5,6). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (IF-IC): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk

Full-length peptide corresponding to human Smac raised in E. coli.

Recognizes endogenous levels of processed and unprocessed human Smac at ~21kD. Reacts weakly with mouse and rat Smac. Species Crossreactivity: Monkey

腹水

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

未确定

Product Type: Mab Isotype: IgG1 Clone No: 4i16 Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Full-length peptide corresponding to human Smac raised in E. coli. Specificity: Recognizes endogenous levels of processed and unprocessed human Smac at ~21kD. Reacts weakly with mouse and rat Smac. Species Crossreactivity: Monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

细胞化学IF免疫组化免疫沉淀免疫印迹

-20°C

1. Du, C. et al. (2000) Cell 102, 33-42. 2. Verhagen, A.M. et al. (2000) Cell 102, 43-53. 3. Srinivasula, S.M. et al. (2001) Nature 410, 112-116. 4. Srinivasula, S.M. et al. (2000) J. Biol. Chem. 275, 36152-36157. 5. Liu, Z. et al. (2000) Nature 408, 1004-1007. 6. Wu, G. et al. (2000) Nature 408, 1008-1012.

https://usbio-images.r.worldssl.net/prodimages/34/S1014-48E_1.jpg

Immubnohistochemical analysis of paraffin-embedded human breast carcinoma, using S1014-48E.

https://usbio-images.r.worldssl.net/prodimages/34/S1014-48E_5.jpg

Immubnohistochemical analysis of paraffin-embedded human colon carcinoma, using S1014-48E.