抗体

Smad2 (MADH2, JV18, MADR2, SMAD2, JV18-1, MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila), Mothers Against Decapentaplegic, Drosophila, Homolog of, 2, MAD (Mothers Against Decapentaplegic, Drosophila) Homolog 2)

S1014-52D

100微克

532美元

多克隆

domestic goat

未共轭

Smad2 (MADH2, JV18, MADR2, SMAD2, JV18-1, MAD, Mothers Against Decapentaplegic Homolog 2 (Drosophila), Mothers Against Decapentaplegic, Drosophila, Homolog of, 2, MAD (Mothers Against Decapentaplegic, Drosophila) Homolog 2)

山羊

多克隆抗体

抗体

100微克

532美元

IgG

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:4000 Western Blot: 1-3ug/ml, ~45kD and 50kD bands seen in HeLa cell lysate Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See S1014-52D-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

Hu

Peptide with sequence SEIWGLSTPNTIDC, from the internal region of the protein sequence according to NP_005892.1. Species sequence homology: bovine

识别人类SMAD家族成员2/MADH2

经免疫亲和层析纯化

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

~0.5毫克/毫升

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Peptide with sequence SEIWGLSTPNTIDC, from the internal region of the protein sequence according to NP_005892.1. Species sequence homology: bovine Specificity: Recognizes Human SMAD2/MADH2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E免疫印迹

-20°C

1. Eppert K, Scherer SW, Ozcelik H, Pirone R, Hoodless P, Kim H, Tsui LC, Bapat B, Gallinger S, Andrulis IL, Thomsen GH, Wrana JL, Attisano L., MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma., Cell 1996 Aug 23; 86(4):543-52

https://usbio-images.r.worldssl.net/prodimages/34/S1014-52D_1.jpg