Tau, phosphorylated (Ser396)
Product wo Prefix :
Tau, phosphorylated (Ser396)
Size1 List :
Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy-terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK-3 and CDK5 (1-2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK-3 or CDK5 destabilizes microtubules in Alzheimer's disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4ºC with gentle shaking, overnight. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu Mo Rt
Human purified Tau (Hoffmann et al., 1997). Species Sequence Homology: human
Recognizes endogenous levels of Tau at 50-80kD only when phosphorylated at serine 396. Species Crossreactivity: mouse and rat.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Product Type: Mab Isotype: IgG2b Clone No: 9B149(PHF13) Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Human purified Tau (Hoffmann et al., 1997). Species Sequence Homology: human Specificity: Recognizes endogenous levels of Tau at 50-80kD only when phosphorylated at serine 396. Species Crossreactivity: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
Hoffmann, R. et al. (1997) Biochemistry 36, 8114–8124. Application: W. Hong, M. and Lee, V.M. (1997) J. Biol. Chem. 272, 19547–19553. Johnson , G.V. and Stoothoff , W.H. (2004) J. Cell Sci. 117, 5721-5729. Hanger, D. P. et al. (1998) J. Neurochem. 71, 2465-2476. Bramblett, G. T. et al. (1993) Neuron 10, 1089-1099.
Picture 1 File Name :
4 Technology Way
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