产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Trypsin discontinued
目录 :
T8669-13A
规格 :
100微升
价格 :
895美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
6F201
更多信息或购买 :
产品信息
目录号 :
T8669-13A
Product wo Prefix :
Trypsin discontinued
宿主 :
小鼠
产品类型 :
单克隆抗体
类别 :
抗体
尺寸1 :
100微升
Size1 List :
895美元
克隆#USB :
6F201
抗体亚型 :
IgG1, k
Desc1 :
Trypsin is a 24kD enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screene for and diagnose Cystic Fibrosis (2). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:4000 Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3. Culture Medium: RPMI 1640 with 10% fetal calf serum Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Bo Hu
免疫原 :
Trypsin purified from human pancreatic tissue (1).
特异性 :
Specific for human trypsin, trypsinogen and trypsin in complex with alpha-1-antitrypsin. Weak crossreactivity with bovine trypsin. Epitope differs from that of T8669-13. Suitable as detection antibody in sandwich ELISA with a coat of T8669-13. Recognizes human trypsin in Western Blot under reducing conditions.
纯度 :
Purified by Protein A/G affinity chromatography.
形式 :
以溶解在PBS液体方式提供, pH值7.4, 0.5M氯化钠, 15mM叠氮化钠
浓度 :
~0.5毫克/毫升
Desc2 :
Product Type: Mab Isotype: IgG1,k Clone No: 6F201 Host: mouse Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.5M sodium chloride, 15mM sodium azide. Purity: Purified by Protein A/G affinity chromatography. Immunogen: Trypsin purified from human pancreatic tissue (1). Specificity: Specific for human trypsin, trypsinogen and trypsin in complex with alpha-1-antitrypsin. Weak crossreactivity with bovine trypsin. Epitope differs from that of T8669-13. Suitable as detection antibody in sandwich ELISA with a coat of T8669-13. Recognizes human trypsin in Western Blot under reducing conditions. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E免疫印迹
储存温度 :
-20°C
参考文献 :
1. Feinstein G, Hofstein R, Koifmann J, Sokolovsky M Human pancreatic proteolytic enzymes and protein inhibitors. Isolation and molecular properties. Eur J Biochem 43:569-581. (1974). 2. Crossley JR, Smith PA, Edgar BW, Gluckman PD, Elliott RB Neonatal screening for cystic fibrosis, using immunoreactive trypsin assay in dried blood spots. Clin Chim Acta 113:111-121.(1981)
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。