产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Tubulin, alpha, acetyl (Lys40) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
目录 :
T9154-01C
规格 :
100微升
价格 :
605美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
10E52(D20G3)
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
T9154-01C
Product wo Prefix :
Tubulin, alpha, acetyl (Lys40) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
宿主 :
产品类型 :
单克隆抗体
类别 :
抗体
尺寸1 :
100微升
Size1 List :
605美元
克隆#USB :
10E52(D20G3)
抗体亚型 :
IgG
Desc1 :
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with alpha/beta-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Gamma-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competi-tive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). The Elongator complex catalytic subunit (Elp3) acetylates alpha-tubulin at Lys40 while the histone deacetylase HDAC6 functions as a tubulin deacetylase. This post-transcriptional modification may be required for dynamic cell shape remod-eling, cell motility, tubulin stability and terminal branching of cortical neurons (2,3). Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:800 Immunoprecipitation: 1:50 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu Mk Mo Rt Ze
免疫原 :
A synthetic acetylpeptide corresponding to residues surrounding Lys40 of human alpha-Tubulin. Species sequence homology: Xenopus
特异性 :
Recognizes endogenous levels of human alpha-Tubulin only when acetylated at Lys40, MW: ~52kD. Species Crossreactivity: mouse, rat, monkey and zebrafish.
纯度 :
纯化
形式 :
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Mab Isotype: IgG Clone No: 10E52(D20G3) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: A synthetic acetylpeptide corresponding to residues surrounding Lys40 of human alpha-Tubulin. Species sequence homology: Xenopus Specificity: Recognizes endogenous levels of human alpha-Tubulin only when acetylated at Lys40, MW: ~52kD. Species Crossreactivity: mouse, rat, monkey and zebrafish. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
IF免疫沉淀免疫印迹
储存温度 :
-20°C
参考文献 :
1. Westermann, S. & Weber, K., Nat. Rev. Mol. Cell Biol. 4: 938-947 (2003). 2. Creppe, C., et al., Cell 136: 551-564 (2009). 3. Hubbert, C., et al., Nature 417: 455-458 (2002).
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/18/T9154-01C_1.jpg
Picture4文本 :
Flow Cytometric analysis of HeLa cells, untreated (blue) or treated with Trichostatin A (TSA) (1uM, 16 hours) using T9154-01C. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor 488 Conjugate) was used as a secondary antibody
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。