抗体

Urokinase Plasminogen Activator (uPA) discontinued

U1820-15

100微升

485美元

单克隆

小鼠

未共轭

9B15

Urokinase Plasminogen Activator (uPA) discontinued

小鼠

单克隆抗体

抗体

100微升

485美元

9B15

IgG1

Urokinase plasminogen activator (uPA) is a serine protease. It converts the abundant proenzyme plasminogen to active plasmin and plays a key role in cancer invasion and a variety of tissue remodelling processes such as wound healing, mammary gland involution and placental development (1-3). Elevated levels of uPA are associated with poor prognosis in many types of cancer (2-5). Applications: Suitable for use in ELISA, Western blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Culture Medium: RPMI 1640 with 10% fetal calf serum Hybridoma: NS1/AG4.1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

天然人类uPA

Recognizes the B-chain of human uPA. Binds single and two-chain uPA, uPA/PAI-1 complex and uPA/uPAR complex (6,7). A reaction is seen with LMW-uPA but not with the amino terminal fragment (ATF). Reacts with non-reduced uPA but not with reduced uPA in Western blotting. It inhibits the enzymatic activity of uPA in the fibrin plate assay and it inhibits the uPA mediated cleavage of uPAR. No reaction with human tissue plasminogen activator (tPA) or with with any other human plasma proteins.

Purified by Protein A affinity chromatography.

以溶解在PBS液体方式提供, pH值7.4, 0.5M氯化钠, 15mM叠氮化钠

~1毫克/毫升

Product Type: Mab Isotype: IgG1 Clone No: 9B15 Host: mouse Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.5M sodium chloride, 15mM sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: Native human uPA Specificity: Recognizes the B-chain of human uPA. Binds single and two-chain uPA, uPA/PAI-1 complex and uPA/uPAR complex (6,7). A reaction is seen with LMW-uPA but not with the amino terminal fragment (ATF). Reacts with non-reduced uPA but not with reduced uPA in Western blotting. It inhibits the enzymatic activity of uPA in the fibrin plate assay and it inhibits the uPA mediated cleavage of uPAR. No reaction with human tissue plasminogen activator (tPA) or with with any other human plasma proteins. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E免疫组化免疫印迹

-20°C

1. Danø K, Andreasen PA, Grøndahl-Hansen J, Kristensen P, Nielsen LS, Skriver L (1985) Plasminogen activators, tissue degradation, and cancer. Adv Cancer Res 44:139-266. 2. Andreasen PA, Kjøller L, Christensen L, Duffey MJ (1997) The urokinase plasminogen activator system in cancer metastasis. Int J Cancer 2:1-22. 3. Danø K, Behrendt N, Høyer-Hansen G, Johnsen M, Lund LR, Ploug M, Rømer J (2005) Plasminogen activation and cancer. Thromb Haem 93:676-681. 4. Harbeck N, Kates RE, Look MP (2002) Enhanced benefit from adjuvant chemotherapy in breast cancer patients classified high-risk acording to urokinase -type plasminogen activator and plasminogen activator inhibitor type 1 (n=3424). Cancae Res 62:4617-22. 5. Grøndahl-Hansen J, Christensen IJ, Rosenquist C, Brünner N, Mouridsen HT, Danø K, Blichert-Toft M (1993) High levels of urokinase-type plasminogen activator and its inhibitor PAI-1 in cytosolic extracts of breast carcinomas are associated with poor prognosis. Cancer Res 53:2513-2521. 6. Kaltoft K, Nielsen LS, Zeuthen J, Danø K (1982) Monoclonal antibody that specifically inhibits a human Mr 52,000 plasminogen-activating enzyme. Proc Natl Acad Sci USA 79:3720-3723. 7. Nielsen LS, Grøndahl-Hansen J, Andreasen PA, Skriver L, Zeuthen J, Danø K (1986) Enzyme linked immunosorbent assay for human urokinase type plasminogen activator and its proenzyme using a combination of monoclonal and polyclonal antibodies. J Immunoassay 7:209-228.