抗体

Vasodilator Stimulated Phosphoprotein (VASP)

V2118-01A

100微升

516美元

多克隆

domestic rabbit

未共轭

Vasodilator Stimulated Phosphoprotein (VASP)

兔

多克隆抗体

抗体

100微升

516美元

IgG

Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP-and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239 and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful tool to monitor PKG activation and signaling (6,7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser157 of human VASP.

Recognizes endogenous levels of total human VASP. Does not crossreact with VASP homologues, such as EVL and Mena. Species crossreactivity: mouse and rat.

Purified by protein A and immunoaffinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

Product Type: Pab Isotype: IgG Host: rabbit Source: human Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol. Purity: Purified by protein A and immunoaffinity chromatography. Immunogen: Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser157 of human VASP. Specificity: Recognizes endogenous levels of total human VASP. Does not crossreact with VASP homologues, such as EVL and Mena. Species crossreactivity: mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫印迹

-20°C

(1) Butt, E. et al. (1994) J. Biol. Chem. 269, 14509–14517. (2) Ball, L.J. et al. (2000) EMBO J. 19, 4903–4914. (3) Machesky, L.M. et al. (2000) Cell 101, 685–688. (4) Smolenski, A. et al. (1998) J. Biol. Chem. 273, 20029–20035. (5) Harbeck, B. et al. (2000) J. Biol. Chem. 275, 30817–30825. (6) Oelze, M. et al. (2000) Circ. Res. 87, 999–1005. (7) Lawrence, D.W. et al. (2001) J. Immunol. 166, 5550–5556.

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