抗体

Vasodilator Stimulated Phosphoprotein, phosphorylated (Ser239) (VASP)

V2118-02B

100微升

566美元

多克隆

domestic rabbit

未共轭

磷酸化

Vasodilator Stimulated Phosphoprotein, phosphorylated (Ser239) (VASP)

兔

多克隆抗体

磷酸化

抗体

100微升

566美元

IgG

Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP-and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance. Three phosphorylation sites, Ser157, Ser239 and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site. Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization. Phosphorylation at Ser239 of VASP is a useful tool to monitor PKG activation and signaling. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic phospho-peptide corresponding to residues surrounding Ser239 of human VASP.

Recognizes endogenous levels of human VASP phosphorylated at serine 239. Does not crossreact with phosphorylated VASP homologues, such as Mena. Species Crossreactivity: mouse, rat and monkey

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

未确定

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser239 of human VASP. Specificity: Recognizes endogenous levels of human VASP phosphorylated at serine 239. Does not crossreact with phosphorylated VASP homologues, such as Mena. Species Crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫印迹

-20°C

1. Butt, E. et al. (1994) J. Biol. Chem. 269, 14509–14517. 2. Ball, L.J. et al. (2000) EMBO J. 19, 4903–4914. 3. Machesky, L.M. et al. (2000) Cell 101, 685–688. 4. Smolenski, A. et al. (1998) J. Biol. Chem. 273, 20029–20035. 5. Harbeck, B. et al. (2000) J. Biol. Chem. 275, 30817–30825. 6. Oelze, M. et al. (2000) Circ. Res. 87, 999–1005. 7. Lawrence, D.W. et al. (2001) J. Immunol. 166, 5550–5556.

https://usbio-images.r.worldssl.net/prodimages/9/V2118-02B_1.jpg