首页
>
试剂
> Cas9
产品
抗CRISPR-Cas9兔单克隆抗体
武汉博士德生物工程有限公司
目录: M30929-1
Western blot analysis of CRISPR-Cas9 expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate (M30929-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cas9 monoclonal antibody (Catalog # M30929-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for cas9
规格: 100 ug/vial
价格: 299美元
至产商
Cas9单克隆抗体(7A9)
赛默飞世尔
目录: MA5-23519
产品总数:
10
文章摘录数: 1
规格: 50µg
价格: 美国390
至产商
Cas9重组兔单克隆抗体(JM11-55)
赛默飞世尔
目录: MA5-32636
产品总数:
10
规格: 100µL
价格: 美国345
至产商
Cas9多克隆抗体
赛默飞世尔
目录: PA5-90171
产品总数:
10
规格: 100µL
价格: 美国345
至产商
CRISPR/Cas9抗体[7A9-3A3]
ImmuQuest
目录: IQ651
规格: 0.1毫升
价格: 345美元
至产商
抗CRISPR-Cas9抗体[EPR19795]
艾博抗(上海)贸易有限公司
目录: ab203943
产品总数:
19
规格: 40微升, 100微升
价格:
至产商
抗CRISPR-Cas9抗体[EPR19799]-BSA和无叠氮物
艾博抗(上海)贸易有限公司
目录: ab218654
产品总数:
19
规格: 100微克
价格:
至产商
抗CRISPR-Cas9抗体[EPR19799](藻红蛋白)
艾博抗(上海)贸易有限公司
目录: ab215701
产品总数:
19
规格: 100微升
价格:
至产商
Cas9单克隆抗体(7A9)
Enzo Life Sciences
目录: ENZ-ABS294-0100
Figure 1. Western blot. HEK293 cells were transiently transfected with pMLM3613myc and lysed in hot Laemmli buffer 40 hours post transfection. Proteins were separated by 7.5% SDS-PAGE and transfered to nitrocellulose. Membranes were blocked in 3% dry milk (NFDM) in PBS-Tween for 1 h at RT and incubated with the indicated antibodies diluted in 0.5% NFDM in PBS-T for 2 hours at RT, followed by incubation with HRP-coupled anti-mouse antibody together with antiBLUE protein marker antibody for 1 hour at RT. Proteins were visualized by ECL.
Figure 2. Immunofluorescence analysis. Hela cells were transiently transfected with an N-terminally Flag-tagged S. pyogenes Cas9 expression vector. 48 hours post transfection cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X and blocked in 2% BSA in PBS for 2 hours at RT. Cells were stained with the indicated antibodies at 4C o/n, followed by incubation with anti mouse-AF488 coupled secondary antibody for 1 h at RT. Nuclei were counter-stained with Hoechst 33342. Images were taken with an Axiovert 135 TV fluorescence microscope. For presentation purposes, images were adjusted for brightness and contrast.
Figure 3. Immunoprecipitation. HEK293T cells expressing N-terminally Flag-tagged S. pyogenes Cas9 were lysed 72 hours post transfection by resuspending the cells in Hunt buffer and subjecting them to three freeze-thaw cycles in liquid nitrogen/on ice. Proteins were immunoprecipitated from 100 ?g of whole cell lysate either for 1 hour at 4°C with crude Cas9 clone 7A9 cell culture supernatant (IgG concentration n.d., lane 3) followed by incubation for 1 hour at 4°C with a 1:1 mixture of protein A/G sepharose beads, or for 2 hours at 4°C with Cas9 clone 7A9 monoclonal antibody crosslinked to a 1:1 mixture of protein A/G sepharose beads. Beads were washed two times with Hunt buffer and once with TBS. Bound proteins were eluted by boiling in Laemmli buffer, separated by 7.5% SDS-PAGE and transfered to nitrocellulose. Membranes were blocked in 3% dry milk (NFDM) in PBS-Tween for 1 h at RT and incubated with Cas9 7A9 monoclonal antibody diluted in 0.5% NFDM in PBS-T for 1 hour at RT, followed by incubation with HRP-coupled anti-mouse antibody. Proteins were visualized by ECL. Asterisk indicates IgG heavy chain.
规格: 100微克
价格: 365美元
至产商
Streptococcus pyogenes CRISPR-associated endonuclease Cas9/Csn1 Antibody (aa1150 ...
LifeSpan Biosciences
目录: LS-C340585
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCAS-Guide vector (GE100002, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAS9.
规格:
价格:
至产商
Cas9(7A9)抗体
圣克鲁斯生物技术
目录: sc-517237
规格: 100微克/毫升
价格: 279美元
至产商
兔多克隆CAS9抗体
北京傲锐东源
目录: TA190309
规格: 100微升
价格: 345美元
至产商
抗CRISPR/Cas9抗体, 小鼠单克隆
西格玛奥德里奇
目录: SAB4200701
文章摘录数: 1
规格:
价格:
至产商
CRISPR/Cas9单克隆抗体
Diagenode
目录: C15200229
产品总数:
2
文章摘录数: 1
规格:
价格:
至产商
pX330-U6-Chimeric_BB-CBh-hSpCas9
Addgene
目录: 42230
产品总数:
69
文章摘录数: 800
规格:
价格:
至产商
微博分享
关注我们的微博
来邦网
英文来邦
其他试剂
兔血清
大鼠血清
钠丁氯倍他松
rifampicin/rifaldazine/RMP/rofact/rifampin
mouse isotype control
rat isotype control
cell viability kit
抗兔IgG Fc抗体
抗小鼠IgG Fc二抗
EGFP
Cimaterol
问题和意见