这是一篇有关细菌表达载体的综述,是根据76篇发表使用实验的文章归纳的。这综述旨在帮助来邦网的访客找到最适合细菌表达载体。
默克密理博中国显示:30; 总数:34
为了研究一种新型磷酸位点结合蛋白- TRAF4 在调节紧密连接和促进细胞迁移中的作用,采用了Novagen的pET28a( +) vector进行克隆。至该文献
为了研究MYRF的自剪切机制,Novagen的pET52b质粒被用于进行DNA克隆实验。至该文献
为了研究动力蛋白激活蛋白(dynactin)在神经细胞微管的作用, 用了Novagen pET-29a来表达人的动力蛋白激活蛋白至该文献
为了研究Nup188和Nup192的结构和功能特性,EMD Millipore的pETDuet-1载体被用于进行DNA克隆实验。至该文献
为了研究alpha-synuclein在诱导突触囊泡类似物聚集中的作用,采用了Novagen的pET28a载体进行基因克隆的实验。至该文献
为了研究RanBP2/Nup358促使的分泌蛋白的翻译,采用了Novagen 公司的pET28a载体来进行RanBP2的克隆实验。至该文献
为了研究阿拉伯芥中具有花粉管引诱剂作用的AtLURE1多肽的进化及其功能,采用了Novagen的表达载体pET-28a(+) 进行相关蛋白的表达实验。至该文献
为了研究cMyBP-C在心肌收缩过程中的作用,采用了Novagen的pET expression system进行蛋白表达实验。至该文献
为了研究MAPK异常活化可被Ste5构象改变隔绝,采用了Novagen的pET15进行蛋白表达实验。至该文献
为了研究大细菌RNA聚合酶钳结构在基因转录过程中的构象改变,采用了Novagen的pET21d进行基因表达实验。至该文献
为了研究隐花色素能够通过特定的小分子激活剂研究,采用了Novagen的pET42b进行蛋白表达实验至该文献
为了研究自组装的蛋白纳米材料可由计算机模拟加以设计,采用了Novagen的pET29b进行质粒构建实验。至该文献
为了研究人源PARP-1功能的结构基础,Novagen的pET28表达载体被用于进行基因克隆。至该文献
为了研究Fam20C参与了胞外蛋白的磷酸化,Novagen的pET28a表达载体被用于进行蛋白表达。至该文献
为了研究YaeJ修复停止的核糖体的机制,采用了Novagen的pET28a vector进行蛋白表达实验。至该文献
为了研究alphaB 晶状体蛋白的结构特点,采用了Novagen的pET15b进行质粒构建实验。至该文献
为了利用cIAP1的自身泛素化能够被拮抗剂诱导的cIAP1的构象改变所促进,采用Novagen的pET-15b进行基因表达实验。至该文献
为了研究功能性蛋白可以通过基于计算机的蛋白骨架嫁接来设计获得,采用Novagen的pET29b vector进行质粒构建实验。至该文献
为了研究Ubc12的N末端乙酰化对它与Dcn1的相互作用至关重要,采用EMD Biosciences的pET-Duet进行蛋白表达实验。至该文献
为了研究细菌中H-NS能够促进染色体的结构改变,采用Novagen的pET30a进行蛋白表达实验。至该文献
为了研究大肠杆菌能够被改造后通过密码子编码磷酸化的丝氨酸,采用Novagen的pET15b进行质粒构建实验。至该文献
为了研究尾锚定蛋白生成过程中Get3-Get1/2受体复合物的动态变化,采用Novagen的pET24d进行载体构建实验。至该文献
为了研究人工设计的RNA模块可以用于细胞内反应的调控,采用Novagen的pETDuet进行载体构建实验。至该文献
为了研究一种计算机模拟设计蛋白的方法可能对流感治疗有用,采用EMD的pET29b expression vector进行蛋白表达实验。至该文献
为了研究甲酸盐载体FocA工作模式的转换,采用Novagen的pET21a载体构建质粒。至该文献
为了研究Norbin在促代谢型谷氨酸受体5信号途径中所起的调控作用,使用了Novagen公司的pET28载体来进行亚克隆。至该文献
为研究E3连接酶RHA2a在种子发芽及幼苗发育中的调节作用,使用了Novagen公司的pET-28a载体扩增His标记的泛素单体。至该文献
为了研究表面活性蛋白A对暴露于肺囊虫环境中的免疫宿主的保护作用,采用了Novagen公司的pET30表达载体,用来构建重组克隆。至该文献
为了说明3′-5′的核酸外切酶活性需要聚合酶和氨醇磷酸酶(PHP)结构域和X家族DNA聚合酶核心(PLOXc)结构域,使用了Novagen公司的pET-11a载体来转载目的基因。至该文献
为了研究寄生虫巨噬细胞移动抑制因子对宿主巨噬细胞所起的调控作用,使用了Novagen公司的pET29质粒来作为表达载体。至该文献
赛默飞世尔
为了研究耶尔森氏菌注射体的结构特点,Invitrogen的pBAD/mycHisA质粒被用于进行DNA克隆。至该文献
为了研究RanBP2/Nup358促使的分泌蛋白的翻译,采用了OpenBiosystems 公司的pSPORT6载体来进行RanBP2的克隆实验至该文献
为了研究拟南芥中DNA去甲基化可被IDM1调控,采用了Invitrogen的pET28a vector进行EMSA实验。至该文献
为了研究carlactone能够通过特定通路由beta胡萝卜素得到,采用了Invitrogen的pBAD/THIO-TOPOTA进行质粒构建实验。至该文献
为了研究以荧光蛋白为基础的钙信号指示剂种类增加对钙成像便捷性的改善,采用Invitrogen的pBAD/His B进行质粒构建实验。至该文献
为了通过晶体结构分析研究驱动蛋白-1的自我抑制是由蛋白尾部结构域与驱动结构域交联所介导,采用Invitrogen的pET28a进行蛋白表达实验。至该文献
为了说明IE1在杆状病毒早期基因复制时作为一种基因选择转录的激活子,使用了英杰公司的pBluescript K/S(+)质粒来转载目的基因。至该文献
Addgene
为了研究钙调神经磷酸酶和CAMKII对秀丽隐杆线虫寿命的调节作用,Addgene的pET15b CnA CnB质粒被用于进行DNA克隆实验。至该文献
New England Biolabs
为了研究碱性磷酸酶催化活性的作用机制,New England Biolabs的pMAL-p2X载体被用于进行蛋白表达。至该文献
为了研究kif2a和importin alpha在非洲蟾蜍胚胎发生时纺锤体缩放中的功能,采用New England Biolabs的pMAL-C5X载体进行DNA克隆试验。至该文献
为了研究carlactone能够通过特定通路由beta胡萝卜素得到,采用了New England Biolabs的pMAL-c4x进行质粒构建实验。至该文献
为了研究alphaB 晶状体蛋白的结构特点,采用了New England Biolabs的pMALC2X进行质粒构建实验。至该文献
为了研究大肠杆菌能够被改造后通过密码子编码磷酸化的丝氨酸,采用New England Biolabs的pMALc2x进行质粒构建实验。至该文献
为了研究植物的天然免疫能被FLS2泛素化抑制,采用New England Biolabs的pMAL-C2进行蛋白表达实验。至该文献
为了研究沙门氏菌的生长被自噬受体optineurin的磷酸化限制,采用NEB的pMALVc2x进行蛋白表达实验。至该文献
为了研究脱镁叶绿酸水解酶和叶片衰老和叶绿素分解相关,采用了New England Biolabs公司的pMAL-c2,进行以pMAL-c2为载体的融合蛋白MBP-DPPH的构建至该文献
为研究E3连接酶RHA2a在种子发芽及幼苗发育中的调节作用,使用了New England Biolabs公司的pMal-c2作为目的基因RHA2a的载体。至该文献
Agilent Technologies
为了研究由植物表皮内特长链脂肪酸合成通过抑制细胞增殖控制的植物器官生长,采用了Stratagene的pBluescript II KS(-) vector构建质粒。至该文献
为了研究昆虫的内共生体可以被抗菌肽段ColA保护,采用Stratagene的pET20b(+)进行蛋白表达实验。至该文献
为研究Ascl1在鼠胚胎端脑中作为Gsx基因的下游效应分子所起的作用,使用了Stratagene公司的pBluescript SK载体来来进行亚克隆。至该文献
为了阐明SAP30L和SAP30在调节染色体重组和转录过程中关键蛋白与蛋白、蛋白与DNA相互作用中的新功能,使用了Stratagene公司的pGEX-4T1克隆全长SAP30L。至该文献
为了说明果蝇的Orc蛋白结合到染色体上需要中断有丝分裂周期蛋白依赖性激酶的活性,使用了Stratagene公司的pBluescript KS(+)来转载目的基因。至该文献
GE Healthcare Life Biosciences显示:30; 总数:32
为了研究果蝇中Matrimony表达水平的调控对于卵母细胞-胚胎过渡的重要性,GE Healthcare的pGEX6p-1质粒被用于进行DNA克隆实验。至该文献
为了研究Myrf对中枢神经系统髓鞘形成的调节作用,GE Healthcare的pGEX-6P-3载体被用于进行DNA克隆。至该文献
为了研究PVRL4在肿瘤进展过程中的作用,Open Biosystems的小鼠pSPORT6-PVRL4被用于进行蛋白质表达实验。至该文献
为了研究alpha-synuclein在诱导突触囊泡类似物聚集中的作用,采用了GE Healthcare的pGEX-KG载体进行蛋白表达实验。至该文献
为了研究二氢硫辛酰胺乙酰基转移酶在基因表达和碳代谢中的双重作用,采用GE Healthcare公司的原核表达载体pGEX4T1进行DNA克隆试验。至该文献
为了研究细菌23S rRNA能通过特定修饰逃避TLR13的识别,采用了GE lifesciences的pGEX2T进行蛋白表达实验至该文献
为了利用单分子成像研究肌动蛋白协作装配的机制,采用了GE Healthcare的pGex-6P1-Vector进行质粒构建实验。至该文献
为了研究p53依赖的细胞增殖和存活能够被atg7调控,采用了GE Healthcare的pGEX4T2 vector进行质粒构建实验。至该文献
为了研究carlactone能够通过特定通路由beta胡萝卜素得到,采用了GE Healthcare的pGEX-5X进行质粒构建实验。至该文献
为了研究拟南芥的免疫反应需要ESD1与免疫调控因子的相互作用且这种相互作用会被致病因子破坏,采用GE Lifesciences的pGEX 4T-3进行蛋白表达实验。至该文献
为了研究ATP浓度可以由磷酸肌醇通过增强糖酵解调控,采用GE Healthcare的pGex-4T-2进行蛋白表达实验。至该文献
为了研究酵母中心体功能受磷酸化调控,采用GE Healthcare的pGEX-6p1进行蛋白表达实验。至该文献
为了研究植物的天然免疫能被FLS2泛素化抑制,采用Pharmacia的pGEX4T-1进行蛋白表达实验。至该文献
为了研究沙门氏菌的生长被自噬受体optineurin的磷酸化限制,采用GE Healthcare的pGEXV4TV1进行蛋白表达实验。至该文献
为了揭示与动物早期进化及动物多样化有关的因子,采用GE Healthcare的改良型pGEX载体构建质粒。至该文献
为了确定人腺病毒的原子结构,使用了GE Healthcare公司的pGEX-KG载体来表达重组蛋白。至该文献
为了研究Norbin在促代谢型谷氨酸受体5信号途径中所起的调控作用,使用了Pharmacia公司的pGEX载体来进行亚克隆。至该文献
为了研究KLF家族成员对中枢神经系统神经元再生能力的调节,使用了Open Biosystems公司的pSPORT载体来进行亚克隆。至该文献
为了研究肿瘤细胞中PI3K途径的激活机制,使用了Amersham公司的pGEX4T-1载体来进行亚克隆。至该文献
为证明β-防卫素-2蛋白质是否是银屑病活动度的血清生物学标志并检测其在皮肤中达到的浓度,用Amersham Pharmacia Biotech提供的pGEX-2T载体来携带人BD-2 PCR扩增产物。至该文献
为了研究yata基因在调控APPL(淀粉前体样蛋白)在细胞内的定位,失去yata 将会诱导神经组织的退化,加速衰老,采用了通用电气医疗公司的pGEX4T-1载体进行了表达一个具有APPL的膜外部分和谷胱甘肽转移酶的融合蛋白实验。至该文献
为了说明HIV-1能够利用imp7来增大其基因组DNA进入细胞核的通道,使用了GE Healthcare公司的pGEX6P3来转载目的基因。至该文献
为了证实蒽环霉素可以抑制HIF-1的转录表达以及肿瘤引起的血管生成细胞的动员,使用了GE Healthcare公司的pGEX-5X-1作为载体来运载HIF-1β的bHLH-PAS结构域。至该文献
GE Healthcare pGEX-4T-3 载体用于克隆dysbindin-1基因cDNA来研究dysbindin-1在中枢神经系统中的生理作用至该文献
为了找出在血管生成过程中铁蛋白的调控作用,研究中使用了安玛西亚的pGEX-6P-1来进行亚克隆。至该文献
为了研究MEN Epsilon或者beta非编码RNA的功能,使用了GE Healthcare公司的pGEX-6P来作为表达载体。至该文献
为研究SUMO修饰CTCF所起的调节作用,使用了Amersham公司的pGEX4T-1来进行亚克隆。至该文献
使用Amersham pGEX-4T-1质粒来得到谷胱甘肽硫转移酶融合蛋白 ,来证明组蛋白泛素化会导致DNA损伤。至该文献
为研究与肌病相关的结蛋白突变对横纹肌肌丝结构的影响,用GE Healthcare提供的pGEX4T-1质粒来携带PCR产物。至该文献
为证实Vg和(或)Sd的结合会影响Dmef2的活性,使用了GE Biotech公司的pGEX-4T1载体来进行亚克隆。至该文献
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